Imbalance between apoptosis and proliferation causes late radiation damage of salivary gland in mouse
Imbalance between apoptosis and proliferation causes late radiation damage of salivary gland in mouse
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DOI：
10.33549/physiolres.930739
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发表时间：
2006-01-01
影响因子：
2.1
通讯作者：
Tomac, J
中科院分区：
文献类型：
--
作者：
Muhvic-Urek, M;Bralic, M;Tomac, J
严重口干症是一种常见的晚期放射后果，发生在头部和颈部恶性肿瘤的照射后。本研究的目的是分析放射治疗后晚期的细胞凋亡和增殖及其相互关系。C57BL/6小鼠头颈部单次照射7.5或15Gy后，分别于照射后40d和90d取颌下腺。采用原位末端标记法和增殖细胞核抗原免疫组织化学方法检测细胞的增殖情况。与90d相比，第40天的组织学变化较轻，当时照射组小鼠的腺体严重萎缩、空泡化和单核细胞浸润。在这两个时间点，受7.5和15Gy射线照射的小鼠腺泡细胞、颗粒细胞和间质导管细胞的凋亡指数均高于未受照射的对照腺体细胞。40d时，仅7.5Gy组颗粒细胞和间质细胞的增殖指数较高。90天时，两个照射组所有细胞类型的增殖指数与对照组相似。根据我们的结果，X射线引起的细胞凋亡和增殖之间的失衡可能是照射后晚期腺体受损的原因。
Severe xerostomia is a common late radiation consequence, which Occurs after irradiation of head and neck malignancies. The aim of the present study was to analyze apoptosis and proliferation and their relationship during the late post-irradiation phase. C57BL/6 mice were locally irradiated in head and neck region with a single dose of 7.5 or 15 Gy and their submandibular glands were collected at 40 and 90 days after irradiation. To identify apoptotic cells, the TUNEL method was employed and immunohistochemistry with proliferating cell nuclear antigen (PCNA) was used for detecting proliferation. Histological changes at day 40 were mild in contrast to day 90 when glands of irradiated mice showed severe atrophy, vacuolization and mononuclear infiltration. Acinar cells, granular and intercalated duct cells of mice irradiated with 7.5 and 15 Gy expressed higher apoptotic index than cells of non-irradiated, control glands at both examined time points. At 40 days, a higher proliferation index in granular and intercalated duct cells was detected only in group irradiated with 7.5 Gy. At 90 days, proliferation index for all cell types in both irradiated groups was similar to the controls. According to our results, the imbalance between apoptosis and proliferation caused by X-irradiation may be the reason for gland impairment during the late post-irradiation phase.