SELECTIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
SELECTIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
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DOI：
10.1128/jcm.15.4.606-609.1982
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发表时间：
1982-01-01
影响因子：
9.4
通讯作者：
SLOTS, J
中科院分区：
文献类型：
--
作者：
SLOTS, J
本文报道了一种用于分离A.伴放线菌TSBV琼脂含有（每升）40 g胰蛋白酶大豆琼脂、1 g酵母提取物、100 ml马血清、75 mg杆菌肽和5 mg万古霉素。TSBV培养基抑制大多数口腔物种，并允许显著较高的A回收率。放线菌共生菌的选择性高于非选择性血琼脂培养基。该菌具有明显的菌落形态和过氧化氢酶阳性反应。放线菌共生菌容易将该细菌与嗜沫嗜血杆菌、二氧化碳嗜纤维菌属（Capnocytophaga spp.）和其他一些污染生物有了TSBV培养基，即使是设备简陋的实验室也能分离和鉴定A。临床（人类）标本中的伴放线菌。
A selective medium, TSBV (tryptic soy-serum-bacitracin-vancomycin) agar, was developed for the isolation of A. actinomycetemcomitans. TSBV agar contained (per l) 40 g of tryptic soy agar, 1 g of yeast extract, 100 ml of horse serum, 75 mg of bacitracin and 5 mg of vancomycin. The TSBV medium suppressed most oral species and permitted significantly higher recovery of A. actinomycetemcomitans than nonselective blood agar medium. The distinct colonial morphology and positive catalase reaction of A. actinomycetemcomitans easily distinguished this bacterium from Haemophilus aphrophilus, Capnocytophaga spp. and a few other contaminating organisms. With the TSBV medium, even modestly equipped laboratories will be able to isolate and identify A. actinomycetemcomitans from clinical (human) specimens.