GROWTH OF AND OMEGA-3-FATTY-ACID PRODUCTION BY PHAEODACTYLUM-TRICORNUTUM UNDER DIFFERENT CULTURE CONDITIONS
GROWTH OF AND OMEGA-3-FATTY-ACID PRODUCTION BY PHAEODACTYLUM-TRICORNUTUM UNDER DIFFERENT CULTURE CONDITIONS
复制标题
DOI：
10.1128/aem.57.2.419-425.1991
复制
发表时间：
1991-02-01
影响因子：
4.4
通讯作者：
WARD, OP
中科院分区：
文献类型：
--
作者：
YONGMANITCHAI, W;WARD, OP
详细研究了氮源、磷酸盐、氯化钠、生长因子、前体物质、CO2、温度、初始pH和接种量对三角褐指藻生物量和二十碳五烯酸（EPA）产量的影响。 总脂肪酸中EPA含量随硝酸盐和尿素浓度的增加而增加。 生长或EPA生产不需要氯化钠。 虽然维生素B1和B12对生长没有显着影响，但通过B12补充，EPA产量增加了65%。 最大的EPA生产发生时，空气充气供应补充有1%的CO2。 EPA生产的最适培养温度和初始pH分别为21.5 - 23 ℃和7.6。 观察到高达133毫克/升培养物的EPA产量。 EPA占总脂肪酸的30 - 40%。
Detailed studies were carried out on the effects of nitrogen source, phosphate, sodium chloride, growth factors, precursors, CO2, temperature, initial pH, and inoculum size on biomass and eicosapentaenoic acid (EPA) production by Phaeodactylum tricornutum. The EPA content of total fatty acids increased with increasing concentrations of nitrate and urea. Sodium chloride was not required for growth or EPA production. While vitamins B1 and B12 did not affect growth significantly, EPA yield was increased by 65% by B12 supplementation. Maximum EPA production occurred when the air gassing supply was supplemented with 1% CO2. Optimum culture temperature and initial pH for EPA production were 21.5 to 23-degrees-C and 7.6, respectively. EPA yields of up to 133 mg/liter of culture were observed. EPA constituted up to 30 to 40% of total fatty acids.