Biomarkers of folate and vitamin B12 are related in blood and cerebrospinal fluid
Biomarkers of folate and vitamin B12 are related in blood and cerebrospinal fluid
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DOI：
10.1373/clinchem.2006.076448
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发表时间：
2007-02-01
影响因子：
9.3
通讯作者：
Herrmann, Wolfgang
中科院分区：
文献类型：
--
作者：
Obeid, Rima;Kostopoulos, Panagiotis;Herrmann, Wolfgang
背景：B族维生素（叶酸，B-12）是脑功能的重要微量营养素和同型半胱氨酸（HCY）代谢的必需辅助因子。HCY增加与神经和精神疾病有关。本实验研究了B族维生素在脑内HCY代谢中的作用。来自72名接受腰椎穿刺的患者的脑脊液（CSF）样本。我们用气相色谱-质谱法测定HCY、甲基丙二酸（MMA）和胱硫醚;用液相色谱串联质谱法测定S-腺苷甲硫氨酸（SAM）和S-腺苷高半胱氨酸（SAH）;用HPLC或免疫测定法测定B族维生素。脑脊液中HCY浓度低于血清或血浆（0.09 vs 9.4 μ mol/L），SAH（13.2 vs 16.8 nmol/L），胱硫醚（54 vs 329 nmol/L）和全转钴胺素CSF中MMA（359对186 nmol/L）和SAM（270对113 nmol/L;均P < 0.05）的浓度较高。脑脊液HCY浓度与脑脊液叶酸（r = -0.46）、脑脊液SAH（r = 0.48）、脑脊液白蛋白（r = 0.31）和年龄（r = 0.32）显著相关。年龄也与较低浓度的CSF-叶酸和较高的CSF-SAH有关。血清叶酸与脑脊液叶酸的相关性取决于血清叶酸水平，当血清叶酸水平<15.7nmol/L时，血清叶酸与脑脊液叶酸的相关系数（r）为0.69。CSF浓度MMA和holotransbobalamin没有显着correlated.Conclusions：CSF和血清/血浆浓度的维生素生物标志物显着相关。年龄越大，CSF-HCY和CSF-SAH越高，CSF-叶酸越低。这些代谢改变可能是低叶酸状态、高同型半胱氨酸血症和神经退行性疾病的重要指标。(c)2007年美国临床化学协会
Background: B-vitamins (folate, B-12) are important micronutrients for brain function and essential cofactors for homocysteine (HCY) metabolism. Increased HCY has been related to neurological and psychiatric disorders. We studied the role of the B-vitamins in HCY metabolism in the brain.Methods: We studied blood and cerebrospinal. fluid (CSF) samples from 72 patients who underwent lumbar puncture. We measured HCY, methylmalonic acid (MMA), and cystathionine by gas chromatography-mass spectrometry; S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) by liquid chromatographytandem mass spectrometry; and the B-vitamins by HPLC or immunoassays.Results: Concentrations were lower in CSF than serum or plasma for HCY (0.09 vs 9.4 mu mol/L), SAH (13.2 vs 16.8 nmol/L), cystathionine (54 vs 329 nmol/L), and holotranscobalamin (16 vs 63 pmol/L), whereas concentrations in CSF were higher for MMA (359 vs 186 nmol/L) and SAM (270 vs 113 nmol/L; all P < 0.05). CSF concentrations of HCY correlated significantly with CSF folate (r = -0.46), CSF SAH (r = 0.48), CSF-albumin (r = 0.31), and age (r = 0.32). Aging was also associated with lower concentrations of CSF-folate and higher CSF-SAH. The relationship between serum and CSF folate depended on serum folate: the correlation (r) of serum and CSF-folate was 0.69 at serum folate < 15.7 nmol/L. CSF concentrations of MMA and holotranscobalamin were not significantly correlated.Conclusions: CSF and serum/plasma concentrations of vitamin biomarkers are significantly correlated. Older age is associated with higher CSF-HCY and CSF-SAH and lower CSF-folate. These metabolic alterations may be important indicators of low folate status, hyperhomocysteinemia, and neurodegenerative diseases. (c) 2007 American Association for Clinical Chemistry