Exploratory and confirmatory gene expression profiling of mac1Δ
Exploratory and confirmatory gene expression profiling of mac1Δ
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DOI：
10.1074/jbc.m212308200
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发表时间：
2004-02-06
影响因子：
4.8
通讯作者：
Vulpe, C
中科院分区：
文献类型：
--
作者：
De Freitas, JM;Kim, JH;Vulpe, C
探索性异常值鉴定方法和验证性基因表达研究表明，在缺乏铜反应转录因子Mac1p的酿酒酵母中诱导了铁调节子。Aft1p/Aft2p结合基序是上调和下调基因之间最具区分性的基序，我们发现了可能受Aft1p/Aft2p调控的新基因。此外，含有铁-S簇的多个编码蛋白的基因下调，表明代谢重组在Mac1Delta中保存铁。每个差异表达基因的零突变都被表征为铜或铁相关的表型。本研究为AKR1、MRS4、PCA1、SSU1、TIS11、YBR047W、YHL035C、YHR045W、YLR047C、YLR126C和YTP1的基因产物在铜和铁的动态平衡中的作用提供了新的或额外的支持。
Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated genes, and we identified new genes potentially regulated by Aft1p/ Aft2p. In addition, multiple genes encoding proteins containing Fe-S clusters were down-regulated suggesting metabolic reorganization to conserve iron in mac1Delta. Null mutants of each of the differentially expressed genes were characterized for copper- or iron-related phenotypes. New or additional support for a role in copper and iron homeostasis is provided in this study for the gene products of AKR1, MRS4, PCA1, SSU1, TIS11, YBR047W, YHL035C, YHR045W, YLR047C, YLR126C, and YTP1.