Investigation on pathogens of major viral diarrhea in pig farms in East China from 2017 to 2019.
Investigation on pathogens of major viral diarrhea in pig farms in East China from 2017 to 2019.
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DOI：
10.11843/j.issn.0366-6964.2020.12.023
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Li, B.
中科院分区：
文献类型：
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作者：
Chang, Xin-jian;Zhou, Jin-zhu;Li, B.
为了解近年来华东地区病毒性腹泻疫情情况，为综合防治提供流行病学调查数据，2017-2019年采集华东地区5市腹泻猪粪便样本549份，采用RT-PCR方法检测猪流行性腹泻病毒（PEDV）、猪轮状病毒（PoRV）、猪传染性胃肠炎病毒（TGEV）、猪三角洲冠状病毒（PDCoV）。并对其阳性率及混合感染情况进行统计分析。结果显示，2017年和2019年期间，PEDV、PDCoV、PoRV、TGEV和PSV阳性率分别为62.6%（372/594）、27.9%（166/594）、16.8%（100/594）、13.3%（79/594）和3.87%（23/594）。感染中，PEDV+PDCoV和PEDV+PoRV的平均混合感染率为主，分别为22.4%（133/594）和13.3%（79/594）。三重感染中，PEDV+PoRV+PDCoV的混合感染率占主导地位，占7.58%（45/594），不存在四、五种病原菌的混合感染。 PEDV阳性检出率最高的是2017年和2019年，以及PoRV和PDCoV的混合感染，是目前华东地区病毒性腹泻防控的重点。 TGEV和PDCoV在健康育肥猪和母猪的检出率中占很大比例，表明隐性感染已成为普遍现象，值得养殖户重视。此外，PSV的检出率也低于其他四种腹泻病毒。总体来看，自2018年8月养殖场加强生物安全防控措施以来，各类病原检出率和场所检出率均呈逐年下降趋势。
To investigate the epidemic situation of viral diarrhea in East China in recent years, and provide epidemiological survey data for comprehensive prevention, 549 fecal samples were collected in 2017-2019 from diarrhea pigs in 5 cities of East China and RT-PCR were used to detect porcine epidemic diarrhea virus (PEDV), porcine rotavirus (PoRV), porcine transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV) and porcine Sapelovirus (PSV), and statistical analysis of its positive rate and mixed infection was performed. The results showed that during 2017 and 2019, PEDV, PDCoV, PoRV, TGEV and PSV positive rate were 62.6% (372/594),27.9% (166/594),16.8% (100/594),13.3% (79/594), and 3.87% (23/594), respectively.In the double mixed infection, the average mixed infection rate of PEDV + PDCoV and PEDV + PoRV were the main ones, which were 22.4% (133/594) and 13.3% (79/594), respectively. Among the triple infections, the mixed infection rate of PEDV + PoRV + PDCoV was dominated by 7.58% (45/594), and there were no mixed infections of four and five pathogens. The highest positive detection rate of PEDV was during 2017 and 2019, and mixed infection with PoRV and PDCoV, which was currently the focus of prevention and control of viral diarrhea in East China. TGEV and PDCoV accounted for a large proportion of the detection rate of healthy fattening pigs and sows, indicating that recessive infections had become common and deserve attention from farmers. In addition, the detection rate of PSV was more lower than the other four diarrhea viruses. On the whole, since August 2018 that the farms strengthened biosecurity prevention and control measures, the detection rate of various pathogens and the detection rate of sites have shown a downward trend year by year.