A biomimetic alternative to poly(ethylene glycol) as an antifouling coating:: Resistance to nonspecific protein adsorption of poly(L-lysine)-graft-dextran
A biomimetic alternative to poly(ethylene glycol) as an antifouling coating:: Resistance to nonspecific protein adsorption of poly(L-lysine)-graft-dextran
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DOI：
10.1021/la800947z
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发表时间：
2008-08-19
期刊：
影响因子：
3.9
通讯作者：
Spencer, Nicholas D.
中科院分区：
文献类型：
--
作者：
Perrino, Chiara;Lee, Seunghwan;Spencer, Nicholas D.
聚（l -赖氨酸）-接枝-葡聚糖（PLL-g-dex）是一种将葡聚糖侧链接枝到聚（l -赖氨酸）骨架上的接枝共聚物，以前被证明可以有效地作为DNA三螺旋的稳定剂和靶向细胞或组织的功能基因的载体，在这项工作中被用来防止蛋白质的非特异性吸附，通过光波导光模光谱测定。PLL-g-dex共聚物很容易从水溶液中吸附到带负电荷的氧化物表面上，并显著减少非特异性蛋白质在裸露的二氧化硅-二氧化钛表面上的吸附。虽然PLL-g-dex共聚物在各种结构下具有有效和等效的表面吸附和防污性能，但原子力显微镜的纳米摩擦学分析能够区分不同的刷密度。
Poly (L-lysine)-graft-dextran (PLL-g-dex), graft copolymers with dextran side chains grafted onto a poly(L-lysine) backbone, previously shown to be effective as stabilizers of DNA triple helices and as carriers of functional genes to target cells or tissues, were employed in this work to prevent nonspecific adsorption of proteins, as determined by means of optical waveguide lightmode spectroscopy. PLL-g-dex copolymers readily adsorb from aqueous solution onto negatively charged oxide surfaces and significantly reduce nonspecific protein adsorption onto bare silica-titania surfaces. While effective and equivalent surface adsorption and antifouling properties were observed for PLL-g-dex copolymers in a variety of architectures, nanotribological analysis by atomic force microscopy was able to distinguish between the different brush densities produced.