Bacterial toxicity of exfoliated black phosphorus nanosheets.
Bacterial toxicity of exfoliated black phosphorus nanosheets.
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DOI：
10.1016/j.ecoenv.2018.06.008
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发表时间：
2018-10
影响因子：
6.8
通讯作者：
Zhiqiang Xiong;Xuejiao Zhang;Siyu Zhang;Lei Lei-Lei;W. Ma;Dengyu Li;Weidong Wang;Qing Zhao;
中科院分区：
文献类型：
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作者：
Zhiqiang Xiong;Xuejiao Zhang;Siyu Zhang;Lei Lei-Lei;W. Ma;Dengyu Li;Weidong Wang;Qing Zhao;
新出现的二维材料黑磷（BP）因其优异的性能在许多领域显示出广阔的应用前景。尽管BP的生物学效应已被研究，但其环境影响尚未得到足够的重视。在这项研究中，首次针对两种模型细菌菌株——革兰氏阴性大肠杆菌（E. coli）和革兰氏阳性枯草芽孢杆菌（B. subtilis）评估了剥离的BP纳米片的细菌毒性。通过监测细菌生长曲线和菌落计数，检查BP纳米片的细菌毒性。对革兰氏阴性E产生更高的毒性。大肠杆菌与革兰氏阳性菌相比 B.枯草芽孢杆菌处理 6 小时后，由于大肠杆菌膜的自修复作用，这种情况在 12 小时时逆转。大肠杆菌。细菌毒性呈时间和浓度依赖性，对大肠杆菌的最大杀菌效率分别为91.65%和99.69%。科利安B.分别是暴露 12 小时后的枯草芽孢杆菌。荧光显微镜、流式细胞术、扫描电子显微镜（SEM）和乳酸脱氢酶（LDH）测定证明，活性氧（ROS）依赖性氧化应激和膜损伤是主要杀菌机制。这项研究表明了 BP 纳米片的潜在环境风险，这项工作的数据将指导其未来的安全应用。
Newly emerged two-dimensional material, black phosphorus (BP) shows promising applications in many fields owing to its superior properties. Despite the biological effects of BP were studied, its environmental impacts have not yet received enough attention. In this study, the bacterial toxicity of exfoliated BP nanosheets was for the first time evaluated against two model bacteria strains, Gram-negativeEscherichia coli(E. coli) and Gram-positive Bacillus subtilis (B. subtilis). By monitoring the bacterial growth curve and colony counting, the bacterial toxicity of BP nanosheets was examined. Higher toxicity was induced for Gram-negativeE. colicompared to Gram-positiveB. subtilisafter 6 h treatment, which was reversed at 12 h due to membrane self-healing ofE. coli. The bacterial toxicity followed a time- and concentration-dependent fashion, with a maximum bactericidal efficiency of 91.65% and 99.69% forE. coliandB. subtilis, respectively, after 12 h exposure. Reactive oxygen species (ROS)-dependent oxidative stress and membrane damage were the main bactericidal mechanisms as proved by fluorescence microscopy, flow cytometry, scanning electron microscopy (SEM), and Lactate dehydrogenase (LDH) assay. This study indicates the potential environmental risk of BP nanosheets and the data from this work will guide their safety applications in the future.