Variation of major neutral oligosaccharides levels in human colostrum
Variation of major neutral oligosaccharides levels in human colostrum
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DOI：
10.1038/sj.ejcn.1602738
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发表时间：
2008-04-01
影响因子：
4.7
通讯作者：
Sanai, Y.
中科院分区：
文献类型：
--
作者：
Asakuma, S.;Urashima, T.;Sanai, Y.
目的：人初乳对保护婴儿免受病原微生物感染具有重要作用。这种保护被认为部分是由于初乳和牛奶中存在的各种中性和酸性寡糖。然而，人初乳中每种寡糖的浓度尚未确定。本研究的目的是确定每个主要的中性低聚糖的浓度连续三天从开始的哺乳期。方法：我们分析了每个中性低聚糖在人类初乳中的水平，连续三天从开始的哺乳期，从12个健康的日本妇女（年龄从21岁到35岁不等;产妇6和multipara 6）。确定供体的ABO血型：A，3; B，3; O，5; AB，1。所测定的人乳低聚糖为2 ′-岩藻糖基乳糖（2 ′-FL）、3-岩藻糖基乳糖（3-FL）、乳二岩藻四糖（LDFT）、乳-N-四糖（LNT）、乳-N-新四糖（LNnT）、乳-N-岩藻五糖（3-LNT）、乳-N-岩藻五糖（LNT）、乳-N-岩藻五（LNFP I、II和III）和两种乳糖-N-二岩藻己糖（LNFDH I和II），使用具有两种衍生化技术的高效液相色谱（HPLC）。第1天初乳中2'-FL和LDFT含量显著高于第2天和第3天（P <0.05）。结论：哺乳期婴儿2'-FL、LDFT和LNT的变化可能反映了哺乳早期婴儿对益生元和抗感染药物的需求。
Objectives: Human colostrum is known to be important for the protection of infants against infection by pathogenic microorganisms. This protection is thought to be due, partially, to various neutral and acidic oligosaccharides that are present in colostrum and milk. However, the concentrations of each of the oligosaccharide of human colostrum have not yet been determined. The aim of this present study was to determine the concentration of each of the major neutral oligosaccharide for three consecutive days from the start of lactation.Method: We analyzed the level of each neutral oligosaccharide in human colostrum, for three consecutive days from the start of lactation, obtained from 12 healthy Japanese women ( ranging in age from 21 to 35 years; primipara 6 and multipara 6). The ABO blood groups of the donors were determined: A, three; B, three; O, five; AB, one. The determined human milk oligosaccharides were 2'- fucosyllactose ( 2'- FL), 3- fucosyllactose ( 3- FL), lactodifucotetraose ( LDFT), lacto- N- tetraose ( LNT), lactoN- neotetraose ( LNnT), three lacto- N- fucopentaose ( LNFP I, II and III) and two lacto- N- difucohexaose ( LNFDH I and II) using high- performance liquid chromatography ( HPLC) with two derivatization techniques.Results: The concentrations of 2'- FL and LDFT in colostrum on day 1 were significantly higher than those on days 2 and 3 ( Po0.05). An increase in LNT was observed on day 3 compared with day 1 ( Po0.05).Conclusion: These changes in concentrations of 2'- FL, LDFT and LNT may reflect the requirements for prebiotics and antiinfection agents by human infants during early lactation.