Maturation and Fertilization of Porcine Oocytes from Primordial Follicles by a Combination of Xenografting and In Vitro Culture1
Maturation and Fertilization of Porcine Oocytes from Primordial Follicles by a Combination of Xenografting and In Vitro Culture1
复制标题
通过异种移植和体外培养相结合使猪原始卵母细胞成熟和受精1
作者：
H. Kaneko;K. Kikuchi;J. Noguchi;M. Hosoe;T. Akita
摘要本研究旨在建立一种使猪原始卵泡卵母细胞完全成熟并具有可移植性的方法，作为大型哺乳动物卵巢异种移植的模型。将20日龄仔猪的卵巢组织移植到切除卵巢的无胸腺小鼠的双肾被膜下，其中大部分卵泡是原始卵泡。宿主小鼠在其阴道涂片中检测到皮质上皮细胞后，用5 IU的马绒毛膜促性腺激素（eCG）处理10天（eCG-10）、30天（eCG-30）或60天（eCG-60）。卵丘-卵母细胞复合体，卵巢移植物，和血液样本，获得后48小时的eCG治疗。移植后45 ~ 70 d，各组宿主小鼠均首次出现阴道角化，并伴有少量有腔卵泡在移植物内形成。然而，我们只从eCG-60组的小鼠中回收了大量的全尺寸卵母细胞;其他组中全尺寸卵母细胞的数量很低。eCG-60组外周总卵泡素水平最高;这支持了我们的发现，即在eCG-60组中，窦状卵泡的生长最增强。在从eCG-60组获得的573个卵母细胞中，98个（17%）在体外培养成熟后处于中期II期。此外，55%的第一极体成熟卵母细胞（n = 20）在体外受精。这些结果清楚地表明，猪原始卵泡卵母细胞的受精是可以实现的异种移植和体外培养的组合。
Abstract Our objective was to develop a method of endowing oocytes from porcine primordial follicles with full maturation and fertilizing ability as a model for ovarian xenografting of large mammals. Ovarian tissues from 20-day-old piglets, in which most of the follicles were primordial, were transplanted under the capsules of both kidneys of ovariectomized athymic mice. The host mice were treated with 5 IU of equine chorionic gonadotropin (eCG) for 10 days (eCG-10), 30 days (eCG-30), or 60 days (eCG-60) after detection of cornified epithelial cells in their vaginal smears. Cumulus-oocyte complexes, ovarian grafts, and blood samples were obtained 48 h after eCG treatment. Forty-five to 70 days after grafting, the host mice in all groups for the first time showed vaginal cornification, accompanied by the formation of a small number of antral follicles in the grafts. However, we recovered large numbers of full-sized oocytes only from mice in the eCG-60 group; the numbers of full-sized oocytes in the other groups were low. Peripheral levels of total inhibin were highest in the eCG-60 group; this supports our finding that the most enhanced growth of antral follicles occurred in this eCG-60 group. Of 573 oocytes obtained from the eCG-60 group, 98 (17%) were at the metaphase II stage after in vitro culture for maturation. Moreover, 55% of matured oocytes with the first polar body (n = 20) were fertilized in vitro. These results clearly demonstrate that fertilization of oocytes from porcine primordial follicles is achievable by a combination of xenografting and in vitro culture.
影响因子：
3.6
作者：
Eppig, JJ;OBrien, MJ
通讯作者：
OBrien, MJ