Association between neuropathic pain characteristics and DNA methylation of transient receptor potential ankyrin 1 in human peripheral blood
Association between neuropathic pain characteristics and DNA methylation of transient receptor potential ankyrin 1 in human peripheral blood
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神经病理性疼痛特征与人外周血瞬时受体电位锚蛋白1 DNA甲基化的关系
DOI：
10.1097/md.0000000000019325
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发表时间：
2020
期刊：
影响因子：
1.6
通讯作者：
Hirose Munetaka
中科院分区：
文献类型：
--
作者：
Takenaka Shiho;Sukenaga Norihiko;Ohmuraya Masaki;Matsuki Yuka;Maeda Lynn;Takao Yumiko;Hirose Munetaka
阐明与人类神经性疼痛相关的表观遗传机制对于预防和治疗这种治疗抗性疼痛状态至关重要。在本研究中，评估了慢性疼痛患者和术前患者的神经病理性疼痛特征与瞬时受体电位锚蛋白1（TRPA 1）基因DNA甲基化之间的关联。对患有慢性疼痛或计划进行胸外科手术的患者的疼痛和心理状态进行前瞻性评估。使用Doubletree Neuropathique 4（DN 4）问卷评估神经病变特征。使用全血检查TRPA 1基因中CpG岛的DNA甲基化水平。本研究入组了48例成人患者。在术前和慢性疼痛患者中，CpG-51的DNA甲基化率的增加与DN 4评分的增加呈正相关。在这些患者中，CpG-51的组合甲基化率也随着DN 4评分的增加而显著增加。神经性疼痛特征可能与人外周血TRPA 1基因启动子区甲基化率相关。
Elucidation of epigenetic mechanisms correlating with neuropathic pain in humans is crucial for the prevention and treatment of this treatment-resistant pain state. In the present study, associations between neuropathic pain characteristics and DNA methylation of the transient receptor potential ankyrin 1 (TRPA1) gene were evaluated in chronic pain patients and preoperative patients. Pain and psychological states were prospectively assessed in patients who suffered chronic pain or were scheduled for thoracic surgery. Neuropathic characteristics were assessed using the Douleur Neuropathique 4 (DN4) questionnaire. DNA methylation levels of the CpG islands in the TRPA1 gene were examined using whole blood. Forty-eight adult patients were enrolled in this study. Increases in DNA methylation rates at CpG-51 showed positive correlations with increases in the DN4 score both in preoperative and chronic pain patients. Combined methylation rates at CpG-51 in these patients also significantly increased together with increase in DN4 scores. Neuropathic pain characteristics are likely associated with methylation rates at the promoter region of the TRPA1 gene in human peripheral blood.