Kinetics of phospholipase A2, arachidonic acid, and eicosanoid appearance in mouse zymosan peritonitis.
Kinetics of phospholipase A2, arachidonic acid, and eicosanoid appearance in mouse zymosan peritonitis.
复制标题
小鼠酵母聚糖腹膜炎中磷脂酶 A2、花生四烯酸和类二十烷酸的动力学。
DOI：
10.4049/jimmunol.144.7.2671
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发表时间：
1990
影响因子：
4.4
通讯作者：
K. Gans
中科院分区：
文献类型：
--
作者：
S. R. Lundy;R. Dowling;T. Stevens;J. Kerr;W. Mackin;K. Gans
腹腔注射酶多糖可诱导小鼠腹膜炎，其特征是细胞内流、血浆渗漏和花生四烯酸（AA）代谢物的出现。我们报道，酶多糖注射也刺激AA、二十二碳六烯酸、亚油酸的积累和磷脂酶A2 （PLA2）的活性。不饱和脂肪酸（UnFA）的量随酵素剂量和时间而变化。注射酶生蛋白15分钟后首次检测到UnFA水平显著升高。酶多糖注射后1 ~ 2 h， UnFA达到最高水平（AA: 725 +/- 29 ng/只，二十二碳六烯酸：296 +/- 23 ng/只，亚油酸：4489 +/- 179 ng/只），8 h后降至生理盐水对照水平。酶多糖注射后5 ~ 15 min， PLA2活性显著升高。PLA2活性的最高水平出现在酶多糖注射后15至30分钟（31.8 +/- 9.1 nmol磷脂/mg蛋白/h），然后在24小时内下降30%。UnFA和PLA2活性的出现与细胞内流无关，但两者与酶多糖注射后5至15分钟的血浆渗出一致。然而，最大渗出发生在注射酶多糖后1至2小时，与UnFA相似，但与PLA2不同。后一种结果表明，在注射酶莫桑的小鼠腹腔中发现的UnFA有很大一部分来自血浆。PLA2在早期时间点（5至15分钟）的活性也可能通过水解组织和/或细胞磷脂来影响UnFA水平。
Intraperitoneal injection of zymosan into mice induces a peritonitis characterized by cellular influx, plasma leakage and the appearance of arachidonic acid (AA) metabolites. We report that zymosan injection also stimulates the accumulation of AA, docosahexaenoic acid, linoleic acid, and phospholipase A2 (PLA2) activity. The amount of the unsaturated fatty acids (UnFA) varies both with the zymosan dose and time. Significantly increased levels of UnFA were first detected 15 min after zymosan injection. Maximal levels of the UnFA were reached 1 to 2 h post zymosan injection (AA: 725 +/- 29 ng/mouse, docosahexaenoic acid: 296 +/- 23 ng/mouse, linoleic acid: 4489 +/- 179 ng/mouse) and declined to saline control levels by 8 h. PLA2 activity was significantly increased 5 to 15 min after zymosan injection. Maximal levels of PLA2 activity occurred 15 to 30 min after zymosan injection (31.8 +/- 9.1 nmol phospholipid/mg protein/h) and then decreased by 30% through 24 h. Neither the appearance of UnFA nor PLA2 activity correlated with cellular influx, but both were coincident with plasma exudation at 5 to 15 min after zymosan. However, maximal exudation occurred 1 to 2 h post zymosan injection similar to that seen with the UnFA but not PLA2. These latter results suggest that a significant portion of the UnFA found in the peritoneal cavity of zymosan-injected mice originates from the plasma. PLA2 activity at the early time points (5 to 15 min) may also contribute to the levels of UnFA via hydrolysis of tissue and/or cellular phospholipids.