DETECTION OF YELLOW FEVER AND DENGUE VIRUSES IN MOSQUITOES BETWEEN 2014 AND 2015 IN BAYELSA AND BENUE STATES OF NIGERIA
DETECTION OF YELLOW FEVER AND DENGUE VIRUSES IN MOSQUITOES BETWEEN 2014 AND 2015 IN BAYELSA AND BENUE STATES OF NIGERIA
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DOI：
10.5281/zenodo.3329978
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Igbinosa, Igho Benjamin
中科院分区：
文献类型：
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作者：
Agwu, Ekenma Julia;Isaac, Clement;Igbinosa, Igho Benjamin
登革热（DEN）和黄热病（YF）疫情以前曾在尼日利亚报告过。为了避免另一次流行，必须在不同地点查明这些病毒的载体。2014年1月至2015年12月期间，在尼日利亚的贝努埃州和巴耶尔萨州，使用人饵法和喷布法在人类住所及其周围捕获雌性成年蚊子，并使用逆转录聚合酶链反应（RT-PCR）检查YF和DEN病毒。总共确定了172，010只成年雌性蚊子，将其放入9，110个蚊子池中，并对YF和DEN病毒进行了测试。对YF和DEN病毒阳性蚊种进行了逐时叮咬活动、真感染率（TIR）和感染蚊密度（DIM）的测定。黄头伊蚊（Aedes luteocephalus，Newstead，1907）、黄头伊蚊（Ae. aegypti（Linnaeus，1762），Ae. cumminsii Theobald，1903，Ae. africanus（Theobald，1901），Ae. albopictus（Skuse，1894），Ae. vittatus（Bigot，1861）、冈比亚按蚊A. nili Theobald，1904，Mansonia africana（Theobald，1901），M. uniformis（Theobald，1901）、Culex annulioris（Theobald，1901）和C. quinquefasciatus（Say，1823），三种蚊种，Ae. luteocephalus，Ae. aegypti和A.在Oju和Ega的样本中检测到YF，而在M. gambiae的样本中检测到DENV-3。来自Ikarama的非洲人阳性蚊种的叮咬方式除白纹伊蚊种群外，均表现为昼夜活动。来自Ega的冈比亚，其通常表现出夜间活动延长至黎明/清晨。AE. aegypti、埃及伊蚊A. gambiae和Ae.在贝努埃州和巴耶尔萨州，黄头绦虫可能是YF的主要传播媒介。M的作用。非洲人传播DENV-3也需要立即调查。
Dengue (DEN) and yellow fever (YF) outbreaks have been previously reported in Nigeria. In order to avoid another epidemic, the identification of the vectors of these viruses at different locations is a necessity. Female adult mosquitoes caught in and around human dwellings in the Benue and Bayelsa States, Nigeria, using human-baiting and spray-sheet methods between January 2014 and December 2015, were examined for YF and DEN viruses using reverse-transcription polymerase chain reaction (RT-PCR). In total 172,010 adult female mosquitoes were identified, put into 9,110 pools of mosquitoes and tested for YF and DEN viruses. The hourly biting activities, true infection rate (TIR) and the density of infected mosquitoes (DIM) were estimated for the species positive to YF and DEN viruses. Among twelve identified species - Aedes luteocephalus (Newstead, 1907), Ae. aegypti (Linnaeus, 1762), Ae. cumminsii Theobald, 1903, Ae. africanus (Theobald, 1901), Ae. albopictus (Skuse, 1894), Ae. vittatus (Bigot, 1861), Anopheles gambiae (Giles, 1902), A. nili Theobald, 1904, Mansonia africana (Theobald, 1901), M. uniformis (Theobald, 1901), Culex annulioris (Theobald, 1901) and C. quinquefasciatus (Say, 1823), three mosquito species, Ae. luteocephalus, Ae. aegypti and A. gambiae, were positive for YF in Oju and Ega, while DENV-3 was detected in samples of M. africana from Ikarama. The biting patterns of these positive mosquito species showed both day and night activities, except the population of A. gambiae from Ega, which typically demonstrated nocturnal activity extended until dawn/early morning hours. Ae. aegypti, A. gambiae and Ae. luteocephalus could represent the primary vectors of YF in the Benue and Bayelsa States. The role of M. africana in transmitting DENV-3 also requires immediate investigation.