Strategies of epithelial repair: modulation of stem cell and transit amplifying cell proliferation.
Strategies of epithelial repair: modulation of stem cell and transit amplifying cell proliferation.
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DOI：
10.1242/jcs.19.111.2867
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发表时间：
1998-01
影响因子：
4
通讯作者：
M. Lehrer;T. Sun;R. Lavker
中科院分区：
文献类型：
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作者：
M. Lehrer;T. Sun;R. Lavker
利用双标记技术，我们研究了仅存在于角膜缘区的角膜上皮干细胞及其后代转运扩增细胞的复制。我们发现角膜上皮干细胞可以通过损伤和TPA诱导进入DNA合成。我们证明了TA细胞层次结构的存在；外周角膜至少要经过两轮DNA合成才能成为有丝分裂后的细胞，而中央角膜仅能进行一轮分裂。然而，这些TA细胞的细胞周期时间可以缩短，这些TA细胞的复制次数可以增加，以应对损伤。因此，这些结果证明了上皮细胞修复的三种策略：(i)干细胞复制，（ii）释放在正常情况下作为未开发储备的额外细胞增殖，以及（iii）通过缩短循环时间来增强TA细胞的增殖。
Using double labeling techniques, we studied the replication of corneal epithelial stem cells that reside exclusively in the limbal zone, and their progeny transit amplifying cells. We show that corneal epithelial stem cells can be induced to enter DNA synthesis by wounding and by TPA. We demonstrate the existence of a hierarchy of TA cells; those of peripheral cornea undergo at least two rounds of DNA synthesis before they become post-mitotic, whereas those of central cornea are capable of only one round of division. However, the cell cycle time of these TA cells can be shortened and the number of times these TA cells can replicate is increased in response to wounding. These results thus demonstrate three strategies of epithelial repair: (i) stem cell replication, (ii) the unleashing of additional rounds of cell proliferation that remain as an untapped reserve under normal circumstances, and (iii) enhancement of TA cell proliferation via a shortening of the cycling time.