A Rat Model of Frozen Shoulder Demonstrating the Effect of Transcatheter Arterial Embolization on Angiography, Histopathology, and Physical Activity
A Rat Model of Frozen Shoulder Demonstrating the Effect of Transcatheter Arterial Embolization on Angiography, Histopathology, and Physical Activity
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DOI：
10.1016/j.jvir.2020.10.017
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发表时间：
2021-02-24
影响因子：
2.9
通讯作者：
Kichikawa, Kimihiko
中科院分区：
文献类型：
--
作者：
Taguchi, Hidehiko;Tanaka, Toshihiro;Kichikawa, Kimihiko
目的：评价经导管动脉栓塞对大鼠冻肩模型的血管造影表现及对身体活动和组织病理学的影响。材料与方法：首先，将肩关节用石膏固定6周的大鼠（n = 4）与正常肩关节未固定的对照大鼠（n = 4）的血管造影和组织病理学结果进行比较。然后将16只冻肩大鼠分为2组。经导管动脉栓塞组（n = 8），左胸肩峰动脉注射亚胺培南/西司他汀。评估手术前后身体活动的变化，并与注射盐水的对照组（n = 8）进行比较。比较两组患者的组织病理学结果。结果：所有冻疮大鼠的血管造影均显示肩关节染色异常。在组织病理学上，关节囊滑膜微血管和单核炎性细胞数量明显高于对照组（P = 0.03）。经导管动脉栓塞组在跑步距离和速度上均有提高（P = 0.03和P = 0.01），而对照组无显著差异。经导管动脉栓塞组微血管数量和单核炎性细胞数量均显著低于对照组（P = 0.002和P = 0.001）。结论：大鼠冻肩模型显示了新生血管的形成。经导管动脉栓塞可减少大鼠冰冻肩的血管数量和炎症变化，增加大鼠的移动距离和速度。
Purpose: To assess the angiographic findings and the effects of transcatheter arterial embolization on physical activity and histopa-thology using a frozen shoulder rat model.Materials and Methods: First, the angiographic and histopathologic findings of rats in which the shoulder was immobilized with molding plaster for 6 weeks (n = 4) were compared to control rats with normal non-immobilized shoulders (n = 4). Next, a total of 16 frozen shoulder rats were divided into 2 groups. In the transcatheter arterial embolization group (n = 8), imipenem/cilastatin was injected into the left thoracoacromial artery. The changes of physical activity before and after procedures were evaluated and compared with a saline-injected control group (n = 8). Histopathologic findings were also compared between the 2 groups.Results: Angiography revealed abnormal shoulder staining in all of the rats with a frozen shoulder. On histopathology, the numbers of microvessels and mononuclear inflammatory cells in the synovial membrane of the joint capsule were significantly higher compared with the control rats (both P = .03). In the transcatheter arterial embolization group, the running distance and speed were improved (P = .03 and P = .01, respectively), whereas there were no significant differences in the control group. The number of microvessels and mononuclear inflammatory cells in the transcatheter arterial embolization group were significantly lower than the control group (P = .002 and P = .001, respectively).Conclusions: The rat frozen shoulder model revealed the development of neovascularization. Transcatheter arterial embolization decreased the number of blood vessels and inflammatory changes in the frozen shoulder and increased the moving distance and speed of the rats.