Nasal provocation with allergen induces a secondary serum IgE antibody response
Nasal provocation with allergen induces a secondary serum IgE antibody response
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DOI：
10.1016/s0091-6749(97)70143-x
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发表时间：
1997-10-01
影响因子：
14.2
通讯作者：
Hamilton, R
中科院分区：
文献类型：
--
作者：
Naclerio, RM;Adkinson, NF;Hamilton, R
季节性抗原暴露的IgE反应的研究受到每年一次的发生和患者暴露于花粉的可变性的限制。为了克服这些问题，我调查了用抗原进行鼻攻击是否会导致血清抗豚草IgE水平升高。我们用纳克数量的豚草抗原提取物鼻内攻击豚草过敏个体，并在攻击前和攻击后每周间隔测量其血清抗豚草IgE水平。在一系列研究中，我们发现，抗原特异性血清IgE水平在攻毒后第一周开始出现可重复的升高，在第四周达到基线水平的约180%，并持续升高8周。并非所有人都表现出这种反应。过敏原特异性IgE对鼻腔激发的反应幅度似乎大于对季节性暴露的反应。攻毒前鼻内倍氯米松给药不影响应答。结果证明了用于研究对变应原的抗原特异性继发性IgE应答的人体内模型。
The study of the IgE response to seasonal antigen exposure is limited by its occurrence once a year and by the variability of patient exposure to pollens. To overcome these problems, me investigated whether nasal challenge with antigen causes an increase in serum anti-ragweed IgE levels. We challenged individuals with ragweed allergy intranasally with nanogram quantities of ragweed antigen extract and measured their serum anti-ragweed IgE levels before and at weekly intervals after challenge. In a series of studies we found that there was a reproducible rise in antigen-specific serum IgE levels beginning the first week after challenge that plateaued at about 180% of baseline levels during the fourth week and remained elevated for 8 weeks. Not all individuals showed this response. The magnitude of the allergen-specific IgE response to nasal challenge appeared to be greater than the response to seasonal exposure. Treatment with intranasal beclomethasone before challenge did not affect the response. The results demonstrate a human in vivo model for the study of the antigen-specific secondary IgE response to allergen.