Penetration of M cells and destruction of Peyer's patches by Yersinia enterocolitica: An ultrastructural and histological study
Penetration of M cells and destruction of Peyer's patches by Yersinia enterocolitica: An ultrastructural and histological study
复制标题
DOI：
10.1099/00222615-44-4-285
复制
发表时间：
1996-04-01
影响因子：
3
通讯作者：
Firsching, R
中科院分区：
文献类型：
--
作者：
Autenrieth, IB;Firsching, R
小肠结肠炎耶尔森菌对人类和啮齿类动物具有肠道致病性，之前的研究提供了证据表明小肠结肠炎耶尔森菌侵入小肠派尔氏淋巴结 (PP) 的淋巴滤泡。在这项研究中，通过扫描（SEM）和透射电子显微镜（TEM）以及常规组织学检查，分析了小肠结肠炎耶尔森氏菌诱导的滤泡相关上皮（FAE）和底层PP组织的组织改变。为此，使用了包括口胃感染以及耳环实验的实验性小鼠感染模型，通过SEM观察了口胃耶尔森菌感染后FAE的快速和选择性定植。 TEM 研究证实，小肠结肠炎耶尔森氏菌紧密粘附在 FAE 上，包括 M 细胞和肠上皮细胞，组织学研究和 TEM 表明，小肠结肠炎耶尔森氏菌选择性地通过 M 细胞而非通过 FAE 的其他细胞侵入 PP，小肠结肠炎耶尔森氏菌感染一天后，FAE 发生改变，在 FAE 下方立即观察到包含表达主要外膜蛋白 YadA 的耶尔森氏菌的小微脓肿，邻近绒毛因淋巴管扩张而扩张，并在上皮内发现游走性多形核白细胞 (PMNL)，感染后 5-7 天，FAE 和部分 PP 被破坏，PP 细胞结构的深刻改变是由于 PMNL 的大量募集，感染后第 5 天，在肠系膜淋巴结中发现脓肿，然而，TEM 研究揭示了小肠结肠炎耶尔森氏菌的证据不仅可以通过淋巴管，还可以通过侵入血管从 PP 传播。总之，本研究的结果表明，FAE 是小肠结肠炎耶尔森氏菌感染中宿主与病原体相互作用的主要部位，并且该病原体穿透 M 细胞，随后诱导 PP 的破坏。
Yersinia enterocolitica is enteropathogenic for man and rodents, Previous studies provided evidence that Y. enterocolitica invades the lymphoid follicles of the Peyer's patches (PP) of the small intestine. In this study Y. enterocolitica-induced tissue alterations of the follicle-associated epithelium (FAE) and the underlying PP tissue were analysed by scanning (SEM) and transmission electron microscopy (TEM) as well as by conventional histological examination, For this purpose, an experimental mouse infection model including orogastric infections as well as peal loop experiments were used, A rapid and selective colonisation of the FAE after orogastric yersinia infection was observed by SEM. TEM studies confirmed that Y. enterocolitica adhered closely to the FAE including M cells and enterocytes, Histological studies and TEM revealed that Y. enterocolitica selectively invaded the PP via M cells but not via other cells of the FAE, One day after Y. enterocolitica infection the FAE was altered and small micro-abscesses comprising yersiniae expressing the major outer-membrane protein YadA were observed immediately beneath the FAE, Adjacent villi were dilated from lymphangiectasis and transmigrating polymorphonuclear leucocytes (PMNL) were found within the epithelium, At 5-7 days after infection the FAE and parts of PP were destroyed, Profound alterations of the cyto-architecture of the PP were due to the enormous recruitment of PMNL, By day 5 after infection, abscesses were found in the mesenteric lymph nodes, However, TEM studies revealed evidence that Y. enterocolitica may disseminate from the PP not only via the lymphatics but also by invasion of blood vessels, Taken together, the results of this study demonstrate that the FAE is the primary site of host-pathogen interaction in Y. enterocolitica infection and that this pathogen penetrates M cells and subsequently induces destruction of the PP.