DIFFERENTIAL ADHERENCE OF HYDROPHOBIC AND HYDROPHILIC CANDIDA-ALBICANS YEAST-CELLS TO MOUSE-TISSUES
DIFFERENTIAL ADHERENCE OF HYDROPHOBIC AND HYDROPHILIC CANDIDA-ALBICANS YEAST-CELLS TO MOUSE-TISSUES
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DOI：
10.1128/iai.59.3.907-912.1991
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发表时间：
1991-03-01
影响因子：
3.1
通讯作者：
CUTLER, JE
中科院分区：
文献类型：
--
作者：
HAZEN, KC;BRAWNER, DL;CUTLER, JE
通过体外结合实验，我们先前证明了在37℃下生长的酵母细胞在小鼠脾脏、淋巴结和肾脏组织中显示出结合特异性。在脾脏和淋巴结组织中，结合主要发生在富含巨噬细胞的区域。在这里，我们测试了疏水和亲水细胞与宿主组织结合的可能性。4株白色念珠菌的疏水和亲水酵母菌细胞与BALB/cByJ小鼠的快速冷冻器官切片在4℃下孵育15 min。通过洗涤去除未附着的细胞，并检查切片。疏水细胞广泛而丰富地与所有组织结合，而亲水细胞则局限于特定部位。例如，疏水细胞结合在脾脏的白髓和红髓以及边缘区，而亲水细胞主要附着在边缘区。疏水酵母细胞附着在整个淋巴结组织，包括皮质旁区域，但亲水细胞附着主要发生在包膜下和小梁窦。EDTA抑制亲水性细胞对小鼠组织的粘附，但对疏水性细胞无抑制作用。疏水性白色念珠菌菌株表现出相似的疏水性，但它们对肾脏和脾脏组织的附着水平在数量上存在差异，这证实了我们之前的观察，即表面疏水性不是粘附宿主细胞的唯一决定因素。其他研究表明，疏水细胞和亲水细胞表现出与其表面性质相关的不同毒力特征，疏水细胞比亲水细胞的毒力更强。综上所述，目前的结果表明，疏水细胞的毒力比亲水细胞强，部分原因可能是疏水细胞在血液清除后结合到各个器官的潜力。
Using an ex vivo binding assay, we previously demonstrated that yeast cells grown at 37-degrees-C display binding specificity in mouse spleen, lymph node, and kidney tissues. In spleen and lymph node tissues, binding was predominantly in regions rich in macrophages. Here, we tested the possibility that hydrophobic and hydrophilic cells bind differentially to host tissues. Hydrophobic and hydrophilic yeast cells of four Candida albicans strains were incubated for 15 min at 4-degrees-C with cryostat sections of organs that had been rapidly frozen after removal from BALB/cByJ mice. Unattached cells were removed by washing, and the sections were examined. Hydrophobic cells bound diffusely and abundantly to all tissues, while hydrophilic cell attachment was restricted to specific sites. For example, hydrophobic cells bound to the white and red pulp and the marginal zones in spleens, whereas hydrophilic cells attached primarily to the marginal zones. Hydrophobic yeast cells attached throughout lymph node tissue including paracortical areas, but hydrophilic cell attachment occurred primarily at the subcapsular and trabecular sinuses. EDTA inhibited the adherence of hydrophilic cells but not hydrophobic cells to mouse tissues. Hydrophobic C. albicans strains displaying similar levels of hydrophobicity differed quantitatively in their levels of attachment to kidney and spleen tissues, confirming our earlier observation that surface hydrophobicity is not the sole determinant in adherence to host cells. Other studies have shown that hydrophobic and hydrophilic cells display different virulence characteristics related to their surface properties and that hydrophobic cells are more virulent than hydrophilic cells. Taken together, the present results suggest that the enhanced virulence of hydrophobic cells over hydrophilic cells may be due, in part, to the potential of hydrophobic cells to bind throughout various organs following clearance from the bloodstream.