Identification of the cDNA, gene and promoter for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia.
Identification of the cDNA, gene and promoter for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia.
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DOI：
10.1016/0965-1748(94)90136-8
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发表时间：
1994-12
影响因子：
3.8
通讯作者：
L. Binger;J. H. Willis
中科院分区：
文献类型：
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作者：
L. Binger;J. H. Willis
我们已经分离并测序了编码HCCP12的基因组DNA的cDNA和区域，HCCP12是在巨蚕Hyalophora cecropia的所有三个变质阶段的柔性表皮中发现的主要蛋白质。HCCP12基因包含两个内含子，第一个内含子中断信号肽。5′侧翼区包含478个碱基对（bp），与常见的Cecropia插入元件的原型具有约95%的同一性。用RACE方法从幼虫、蛹和成虫表皮细胞中扩增HCCP 12基因5′端的cDNA。21个克隆的cDNA序列几乎完全相同。因此得出结论，HCCP 12的单个基因和单个启动子（如由单个转录起始位点证明的）用于所有三个变质阶段。因此，无论是阶段特异性基因集，也不是阶段特异性启动子是必不可少的表皮蛋白基因表达的变态转变。
We have isolated and sequenced a cDNA and region of genomic DNA that encode HCCP12, a major protein found in flexible cuticles of all three metamorphic stages of the giant silkmoth,Hyalophora cecropia. The gene for HCCP12 contains two introns with the first intron interrupting the signal peptide. The 5′ flanking region contains 478 base pairs (bp) with about 95% identity to the prototype of a common Cecropia insertion element. The RACE procedure was used to produce cDNAs corresponding to the 5′ ends of mRNAs for HCCP12 isolated from larval, pupal and pharate adult epidermal cells. Sequences from 21 cloned cDNAs were almost identical. It was thus concluded that a single gene and a single promoter (as evidenced by a single transcriptional start site) for HCCP12 are used in all three metamorphic stages. Hence, neither stage-specific gene sets nor stage-specific promoters are essential for metamorphic transitions of cuticular protein gene expression.