Multinucleated giant cells in various forms of giant cell containing lesions of the jaws express features of osteoclasts
Multinucleated giant cells in various forms of giant cell containing lesions of the jaws express features of osteoclasts
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DOI：
10.1034/j.1600-0714.2003.00126.x
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发表时间：
2003-07-01
影响因子：
3.3
通讯作者：
Li, TJ
中科院分区：
文献类型：
--
作者：
Liu, B;Yu, SF;Li, TJ
背景：颌骨各种含巨细胞病变中多核巨细胞（MGC）形成的性质和机制尚不完全清楚。本研究的目的是阐明中央巨细胞肉芽肿 (CGCG)、外周巨细胞肉芽肿 (PGCG)、天使性和动脉瘤性骨囊肿 (ABC) 中 MGC 的破骨特征，以及 MGC 形成中细胞成分之间相互关系的机制。 方法：使用一组抗体进行免疫组织化学研究，包括空泡 H+ -ATPase (V-ATPase)、碳酸酐酶 II (CA) II)、组织蛋白酶 K、基质金属蛋白酶-9 (MMP-9)、CD68、增殖细胞核抗原 (PCNA) 以及抗酒石酸酸性磷酸酶 (TRAP) 酶组织化学染色，对 CGCG (n = 34)、PGCG (n = 6)、cherubism (n = 7) 和 ABC (n = 6).还进行了原位杂交检测这四种病变中NF-κB配体受体激活剂（RANKL）（一种新发现的在破骨细胞生成中至关重要的细胞因子）及其受体RANK（NF-κB配体受体激活剂）及其诱饵受体OPG（骨保护素）的mRNA表达。 结果：免疫组化和酶组织化学研究表明，这些病变中的 MGC 和部分单核细胞呈 TRAP、V-ATPase、CA II、组织蛋白酶 K、MMP-9 和 CD68 强阳性，而纺锤形单核细胞则 PCNA 呈阳性。原位杂交结果表明，RANKL mRNA主要表达于纺锤体单核细胞，而OPG广泛分布于MGC和单核细胞中。 RANK mRNA在MGC和一些圆形单核细胞中表达。结论：这些结果表明，四种类型的颌骨巨细胞病变中的MGC表现出破骨细胞表型的特征。 TRAP呈阳性的单核基质细胞可能是MGC的前体细胞。这些病变中表达的 RANKL、OPG 和 RANK 可能在 MGC 的形成中发挥重要作用。这些病变中MGCs分化的相似特征和机制也表明这些病变中MGCs的形成可能存在相似的发病机制。
Background: The nature and the mechanism involved in the formation of the multinucleated giant cells (MGCs) in various giant cell-containing lesions of the jaws are not fully understood. The aim of this study is to clarify the osteoclastic features of the MGCs in central giant cell granuloma (CGCG), peripheral giant cell granuloma (PGCG), cherubism, and aneurysmal bone cyst (ABC), and the mechanism underlying the interrelations between cellular components in the formation of the MGCs.Methods: Immunohistochemical study with a panel of antibodies including vacuolar H+ -ATPase (V-ATPase), carbonic anhydrase II (CA II), Cathepsin K, matrix metalloproteinases-9 (MMP-9), CD68, and proliferating cell nuclear antigen (PCNA), and enzyme histochemical staining for tartarate-resistant acid phosphatase (TRAP) were applied on a total number of 53 cases of giant cell-containing lesions including CGCG (n = 34), PGCG (n = 6), cherubism (n = 7), and ABC (n = 6). In situ hybridization was also carried out to detect the mRNA expression of the receptor activator of NF-kappaB ligand (RANKL), a newly identified cytokine that is shown to be essential in the osteoclastogenesis, its receptor RANK (receptor activator of NF-kappaB ligand), and its decoy receptor OPG (osteoprotegerin) in these four types of lesions.Results: Immunohistochemical and enzyme histochemical studies showed that both the MGCs and a fraction of mononuclear cells in these lesions were strongly positive for TRAP, V-ATPase, CA II, Cathepsin K, MMP-9, and CD68, while the spindle-shaped mononuclear cells were positive for PCNA. The results with in situ hybridization indicated that RANKL mRNA was mainly expressed in the spindle mononuclear cells while OPG was extensively distributed in both the MGCs and the mononuclear cells. RANK mRNA was expressed in the MGCs and some round mononuclear cells.Conclusions: These results suggest that MGCs in the four types of giant cell-containing lesions of the jaws show characteristics of the osteoclast phenotype. The mononuclear stromal cells, which show TRAP positively, may be the precursors of the MGCs. RANKL, OPG, and RANK expressed in these lesions may play important roles in the formation of the MGCs. The similar characteristics and mechanisms in the differentiation of MGCs in these lesions also suggest that there might be a similar kind of pathogenesis involved in the formation of the MGCs in these lesions.