[Comparison of Soil Bacterial Community Structure Between Paddy Fields and Dry Land in the Huixian Karst Wetland, China].
[Comparison of Soil Bacterial Community Structure Between Paddy Fields and Dry Land in the Huixian Karst Wetland, China].
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DOI：
10.13227/j.hjkx.201811048
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发表时间：
2019-07-08
期刊：
影响因子：
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通讯作者：
Liu, De-Shen
中科院分区：
文献类型：
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作者：
Jia, Yuan-Hang;Jin, Zhen-Jiang;Liu, De-Shen
为探讨土地利用变化对湿地系统土壤细菌的影响，采集了辉县喀斯特湿地自然湿地（NW）、水田（PF）和旱地（DL）的表层土壤（0-20 cm）。利用高通量测序技术分析了土壤细菌群落的α多样性、物种组成和丰度。还研究了环境因素对细菌群落结构的影响。结果表明，辉县岩溶湿地土壤细菌可分为49门145纲。细菌的香农指数在PF显着较高，而在西北部的细菌的辛普森指数显着较低，比其他两种土地利用类型。优势门西北部（操作分类单位，OTUs>1%）为变形菌门（52.15%），放线菌（15.16%）和酸杆菌属（8.80%），其中变形菌门为优势门结果表明，该水域的优势类群为变形杆菌属（51.42%）、酸杆菌属（15.51%）和绿弯杆菌属（7.43%）。西北部优势类群（OTU>1%）为α-变形菌门（17.98%），β-变形菌门（13.72%）和放线菌（13.13%），优势类群为酸杆菌（14.35%），β-变形菌门变形菌门（13.37%）和三角变形菌门（12.02%），优势类群为α变形菌门（19.44%）、变形菌门（13.30%）和酸杆菌门（13.03%）。在占主导地位的OTU中，（>0.3%），西北部优势菌属为鞘氨醇单胞菌属（OTU 2，59），小单孢菌属（OTU 5、24和50487）、芽单胞菌属（OTU 1）和营养单胞菌属（OTU 8）; PF中的优势属为溶杆菌属优势菌属为鞘氨醇单胞菌属（OTU 85、157和2916）、罗丹明菌属（OTU 19和52）和隔杆菌属（OTU 60）。热图显示，3种土地利用类型土壤细菌群落结构存在显著差异。结果表明，pH值、土壤有机碳（SOC）、全氮（TN）、碱解氮（AN）、交换性镁离子（Mg 2+）、交换性钙离子（Ca 2+）、可溶性有机碳（DOC）和有效磷（AP）是影响辉县岩溶湿地细菌群落结构的主要因子。这些结果表明，土地利用类型的变化显着塑造了该地区的土壤细菌群落结构。
In order to explore the effect of land-use change on soil bacteria in wetland systems, the topsoil (0-20 cm) of a natural wetland (NW), paddy field (PF), and dry land (DL) were collected in the Huixian karst wetland. The alpha-diversity, species composition, and abundance of soil bacterial communities were analyzed using high-throughput sequencing. The effect of environmental factors on bacterial community structure was also examined. The results showed that the soil bacteria in the Huixian karst wetland can be divided into 49 phyla and 145 classes. The Shannon index of bacteria in the PF was significantly higher, and the Simpson index of bacteria in the NW is significantly lower, than in the other two land-use types. The dominant phyla (operational taxonomic units, OTUs>1%) in the NW were Proteobacteria (52.15%), Actinobacteria (15.16%), and Acidobacteria (8.80%); the dominant phyla in the PF were Proteobacteria (45.79%), Acidobacteria (17.20%), and Chloroflexi (11.75%); the dominant phyla in the DL were Proteus (51.42%), Acidobacteria (15.51%), and Chloroflexi (7.43%). The dominant classes (OTUs>1%) in the NW were alpha-Proteobacteria (17.98%), beta-Proteobacteria (13.72%), and Actinobacteria (13.13%); the dominant classes in the PF were Acidobacteria (14.35%), beta-Proteobacteria (13.37%), and delta-Proteobacteria (12.02%); the dominant classes in the DL were alpha-Proteobacteria (19.44%), Formobacteria (13.30%), and Acidobacteria (13.03%). Among the dominant OTUs (>0.3%), the dominant genera of in the NW were Sphingomonas (OTU2, 59), Micromonospora (OTU5, 24 and 50487), Gemmatimonas (OTU1), and Tenotrophomonas (OTU8); the dominant genera in the PF were Lysobacter (OTU4 and 115) and Aquabacterium (OTU33); the dominant genera in the DL were Sphingomonas (OTU85, 157 and 2916), Rhodanobacter (OTU19 and 52), and Penlobacterium (OTU60). A heatmap showed that there were significant differences in soil bacterial community structure among the three land-use types. Redundancy analysis showed that pH, soil organic carbon (SOC), total nitrogen (TN), alkali-hydrolyzable nitrogen (AN), exchangeable Mg2+, exchangeable Ca2+, soluble organic carbon (DOC), and available phosphorus (AP) were the main factors that affected the bacterial community structure in the Huixian karst wetland. These results indicate that changes in land-use types have significantly shaped the structure of soil bacterial communities in this area.