Dec1 and Dec2 expression is disrupted in the suprachiasmatic nuclei of Clock mutant mice
Dec1 and Dec2 expression is disrupted in the suprachiasmatic nuclei of Clock mutant mice
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DOI：
10.1177/0748730403262870
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发表时间：
2004-04-01
影响因子：
3.5
通讯作者：
Honma, K
中科院分区：
文献类型：
--
作者：
Butler, MP;Honma, S;Honma, K
DEC 1和DEC 2是基本的螺旋-环-螺旋转录因子，功能上类似于哺乳动物昼夜节律钟的负反馈成分。Dec 1和Dec 2基因在大鼠视交叉上核中有节奏地表达，Dec 1的表达以时间依赖性的方式受到光的刺激，具有立即早期基因的动力学。DEC 1和DEC 2可以抑制时钟基因Per 1的CLOCK：BMAL 1反式激活，这表明这些转录因子可能有助于调节昼夜节律。作者提供了野生型和纯合Clock突变小鼠中Dec 1和Dec 2表达模式的数据。在视交叉上核中，Clock突变显著降低了Dec 1和Dec 2的表达。12月1日成为光周期; 12月2日在12 L：12 D光暗周期中保持弱节奏，但在恒定的黑暗中是光周期。在检查的所有脑区中检测到Clock突变小鼠中Dec 1和Dec 2信号的稳健衰减。这些数据表明Clock在体内上调了Dec 1和Dec 2。
DEC1 and DEC2 are basic helix-loop-helix transcription factors that functionally resemble negative feedback components of the mammalian circadian clock. The genes Dec1 and Dec2 are expressed rhythmically in the rat suprachiasmatic nuclei, and Dec1 expression is stimulated by light in a time-dependent manner with the kinetics of an immediate early gene. DEC1 and DEC2 can inhibit CLOCK:BMAL1 transactivation of the clock gene Per1, suggesting that these transcription factors may help regulate circadian timing. The authors present data on the expression pattern of Dec1 and Dec2 in wild-type and homozygous Clock mutant mice. In the suprachiasmatic nuclei, the Clock mutation significantly reduces the expression of Dec1 and Dec2. Dec1 becomes arrhythmic; Dec2 remains weakly rhythmic in a 12L:12D light-dark cycle but is arrhythmic in constant darkness. A robust attenuation of the Dec1 and Dec2 signals in Clock mutant mice was detected in all brain areas examined. These data point to up-regulation of Dec1 and Dec2 by Clock in vivo.