RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo
RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo
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DOI：
10.1038/ng.2007.26
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发表时间：
2007-12-01
期刊：
影响因子：
30.8
通讯作者：
Young, Richard A.
中科院分区：
文献类型：
--
作者：
Zeitlinger, Julia;Stark, Alexander;Young, Richard A.
人们普遍认为，基因激活的关键限速步骤是RNA聚合酶II（Pol II）募集到核心启动子（1）。虽然有充分记载的例子，其中Pol II被募集到基因中但停滞（2-12），但Pol II停滞在发育中的一般作用尚未确定。我们在果蝇胚胎中进行了全面的Pol II染色质免疫沉淀微阵列（ChIP芯片）测定，并确定了三种不同的Pol II结合行为：主动（整个转录单位的均匀结合），无结合，和停滞（在转录起始位点结合）。接近10%的停滞基因的显著特征是它们高度富集发育控制基因，这些基因在胚胎发生的后期阶段被抑制或准备激活。我们建议，Pol II失速促进快速的时间和空间变化的基因活性在发展过程中。
It is widely assumed that the key rate-limiting step in gene activation is the recruitment of RNA polymerase II ( Pol II) to the core promoter(1). Although there are well-documented examples in which Pol II is recruited to a gene but stalls(2-12), a general role for Pol II stalling in development has not been established. We have carried out comprehensive Pol II chromatin immunoprecipitation microarray ( ChIP-chip) assays in Drosophila embryos and identified three distinct Pol II binding behaviors: active ( uniform binding across the entire transcription unit), no binding, and stalled ( binding at the transcription start site). The notable feature of the similar to 10% genes that are stalled is that they are highly enriched for developmental control genes, which are either repressed or poised for activation during later stages of embryogenesis. We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development.