Distribution and bioinformatic analysis of the cerato-platanin protein family in Dikarya
Distribution and bioinformatic analysis of the cerato-platanin protein family in Dikarya
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DOI：
10.3852/13-115
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发表时间：
2013-11-01
期刊：
影响因子：
2.8
通讯作者：
Asiegbu, Fred O.
中科院分区：
文献类型：
--
作者：
Chen, Hongxin;Kovalchuk, Andriy;Asiegbu, Fred O.
Cerato-Platanin家族是一类新发现的富含半胱氨酸的小分子真菌蛋白。它们通常在细胞外大量分泌，并参与真菌与宿主的相互作用。随着现有真菌基因组序列的发展，我们对该家族在真菌中的分布进行了全基因组研究，并分析了蛋白质序列的共同特征。总共使用了55个真菌基因组，其中27个来自子囊菌门，28个来自担子菌门。共获得了130个Cerato-Platanin同源蛋白序列并进行了分析。我们的结果表明，Cerato-Platanin同系物在子囊菌门和担子菌门中都存在，但在果冻真菌的早期分支中以及在生活史中以酵母或酵母样形式存在的一些类群中缺失。子囊菌门和担子菌门的同源基因数目有很大差异。系统发育分析表明，Dikarya的祖先具有多个Cerato-Platanins拷贝，这种拷贝在子囊菌门和担子菌门中的排序不同，该基因家族可能在担子菌门中扩大了。几乎所有的同源物都含有信号肽序列，成熟蛋白的长度主要是105-134个氨基酸。参与形成两个二硫键和签名序列(CSD或CSN)的四个半胱氨酸在大多数同源物中高度保守。这些结果表明担子菌门Cerato-Platanin家族的多样性高于子囊菌门。
The cerato-platanin family is a group of small cysteine-rich fungal proteins new to science. They usually are abundantly secreted extracellularly and are involved in fungus-host interactions. With the advance of available fungal genome sequences, we performed a genomewide study of the distribution of this family in fungi and analyzed the common characteristics of the protein sequences. A total of 55 fungal genomes, including 27 from Ascomycota and 28 from Basidiomycota, were used. A total of 130 cerato-platanin homolog protein sequences were obtained and analyzed. Our results showed that cerato-platanin homologs existed in both Ascomycota and Basidiomycota but were lost in early branches of jelly fungi as well as in some groups with yeast or yeast-like forms in their life cycle. Homolog numbers varied considerably between Ascomycota and Basidiomycota. Phylogenetic analysis suggested that the ancestor of the Dikarya possessed multiple copies of cerato-platanins, which sorted differently in Ascomycota and Basidiomycota, and that this gene family might have expanded in the Basidiomycota. Almost all homologs contained signal peptide sequences, and the length of mature proteins were mainly 105-134 amino acids. Four cysteines involved in forming two disulfide bridges and signature sequences (CSD or CSN) were highly conserved in most homologs. These results indicated a higher diversity of the cerato-platanin family in Basidiomycota than Ascomycota.