Quantitative modeling of sensitivity in bacterial chemotaxis: The role of coupling among different chemoreceptor species
Quantitative modeling of sensitivity in bacterial chemotaxis: The role of coupling among different chemoreceptor species
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DOI：
10.1073/pnas.1330839100
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发表时间：
2003-07-08
影响因子：
11.1
通讯作者：
Tu, YH
中科院分区：
文献类型：
--
作者：
Mello, BA;Tu, YH
我们提出了一个一般的理论框架，考虑到受体的相互作用，包括不同的受体物种之间的受体敏感性在细菌趋化性建模。我们表明，我们的模型可以定量地解释最近在不同的配体浓度的突变体和野生型菌株的受体敏感性的体内测量。对于突变菌株，我们的模型可以准确地拟合实验数据。对于野生型细胞，我们的模型能够实现高增益，同时具有适度的希尔系数值的响应曲线。此外，在我们的模型中的野生型细胞的高灵敏度保持了广泛的环境配体浓度，促进了近乎完美的适应和受体活性的配体结合的依赖性。我们的研究揭示了不同化学感受器物种之间耦合的重要性，特别是天冬氨酸（焦油）和丝氨酸（Tsr）受体之间的强相互作用，这对于解释突变体和野生型数据至关重要。其他突变株的敏感性和可能的改进，我们的野生型细胞模型的预测也进行了讨论。
We propose a general theoretical framework for modeling receptor sensitivity in bacterial chemotaxis, taking into account receptor interactions, including those among different receptor species. We show that our model can quantitatively explain the recent in vivo measurements of receptor sensitivity at different ligand concentrations for both mutant and wild-type strains. For mutant strains, our model can fit the experimental data exactly. For the wild-type cell, our model is capable of achieving high gain while having modest values of Hill coefficient for the response curves. Furthermore, the high sensitivity of the wild-type cell in our model is maintained for a wide range of ambient ligand concentrations, facilitated by near-perfect adaptation and dependence of ligand binding on receptor activity. Our study reveals the importance of coupling among different chemoreceptor species, in particular strong interactions between the aspartate (Tar) and serine (Tsr) receptors, which is crucial in explaining both the mutant and wild-type data. Predictions for the sensitivity of other mutant strains and possible improvements of our model for the wild-type cell are also discussed.