Fluorine-18-labeled phospholipid quantum dot micelles for in vivo multimodal imaging from whole body to cellular scales
Fluorine-18-labeled phospholipid quantum dot micelles for in vivo multimodal imaging from whole body to cellular scales
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DOI：
10.1021/bc800179j
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发表时间：
2008-09-01
影响因子：
4.7
通讯作者：
Tavitian, Bertrand
中科院分区：
文献类型：
--
作者：
Duconge, Frederic;Pons, Thomas;Tavitian, Bertrand
我们设计了适用于正电子发射断层扫描（PET）和光学荧光体内成像的新纳米探针。通常用于临床成像的氟-18已经偶联到磷脂量子点（QD）胶束。将该探针注射到小鼠体内，我们证明了其动态定量全身生物分布和药代动力学可以使用PET以及使用体内纤维共聚焦荧光成像的细胞摄取动力学进行监测。QD的磷脂胶束包封提供了高度通用的表面化学，以使多种化学品和生物分子与受控的QD：分子价缀合。在这里，我们表明，与以前的几项研究相比，使用其他QD聚合物涂层，这些磷脂QD胶束表现出较长的循环半衰期在血流中（约2小时）和缓慢的网状内皮系统的吸收。
We have designed new nanoprobes applicable for both positron emission tomography (PET) and optical fluorescence in vivo imaging. Fluorine-18, which is commonly used for clinical imaging, has been coupled to phospholipid quantum dot (QD) micelles. This probe was injected in mice and we demonstrated that its dynamic quantitative whole body biodistribution and pharmacokinetics could be monitored using PET as well as the kinetics of their cellular uptake using in vivo fibered confocal fluorescence imaging. Phospholipid micelle encapsulation of QDs provides a highly versatile surface chemistry to conjugate multiple chemicals and biomolecules with controlled QD:molecule valency. Here, we show that, in contrast with several previous studies using other QD polymer coatings, these phospholipid QD micelles exhibit long circulation half-time in the bloodstream (on the order of 2 h) and slow uptake by reticulo-endothelial system.