NEOCARTILAGE FORMATION INVITRO AND INVIVO USING CELLS CULTURED ON SYNTHETIC BIODEGRADABLE POLYMERS
NEOCARTILAGE FORMATION INVITRO AND INVIVO USING CELLS CULTURED ON SYNTHETIC BIODEGRADABLE POLYMERS
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DOI：
10.1002/jbm.820270104
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发表时间：
1993-01-01
期刊：
影响因子：
--
通讯作者：
LANGER, R
中科院分区：
文献类型：
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作者：
FREED, LE;MARQUIS, JC;LANGER, R
使用生长在合成的、可生物降解的聚合物支架上的软骨细胞创建了可能用于重建或整形外科手术的软骨植入物。将从牛或人关节或肋软骨分离的软骨细胞培养在纤维状聚乙醇酸（PGA）和多孔聚（L）乳酸（PLLA）上，并平行用于体外和体内研究。以一定时间间隔采集样本，用于评估细胞数量和软骨基质（硫酸化糖胺聚糖[S-GAG]、胶原蛋白）。软骨细胞分泌软骨基质以填充同时生物降解的聚合物支架中的空隙空间。在体外，在PGA上生长6周的软骨细胞达到5.2 × 10（7）个细胞/g的细胞密度，是第1天的8.3倍，与正常牛关节软骨的细胞密度相等。在体外，PGA上的细胞生长速率约为PLLA上的两倍;在PGA上生长的细胞以高稳定速率产生S-GAG，而在PLLA上生长的细胞仅产生极少量的S-GAG。这些差异可归因于聚合物的几何形状和生物降解速率。在体内，在PGA和PLLA上生长1-6个月的软骨细胞保持了原始聚合物支架的三维（3-D）形状，宏观上呈现出闪亮的白色，含有S-GAG和II型胶原，在组织学上与软骨非常相似。这些研究证明了在生物可降解聚合物支架上培养分离的软骨细胞以再生3-D新软骨的可行性。
Cartilaginous implants for potential use in reconstructive or orthopedic surgery were created using chondrocytes grown on synthetic, biodegradable polymer scaffolds. Chondrocytes isolated from bovine or human articular or costal cartilage were cultured on fibrous polyglycolic acid (PGA) and porous poly(L)lactic acid (PLLA) and used in parallel in vitro and in vivo studies. Samples were taken at timed intervals for assessment of cell number and cartilage matrix (sulfated glycosaminoglycan [S-GAG], collagen). The chondrocytes secreted cartilage matrix to fill the void spaces in the polymer scaffolds that were simultaneously biodegrading. In vitro, chondrocytes grown on PGA for 6 weeks reached a cell density of 5.2 X 10(7) cells/g, which was 8.3-fold higher than at day 1, and equalled the cellularity of normal bovine articular cartilage. In vitro, the cell growth rate was approximately twice as high on PGA as it was on PLLA; cells grown on PGA produced S-GAG at a high steady rate, while cells grown on PLLA produced only minimal amounts of S-GAG. These differences could be attributed to polymer geometry and biodegradation rate. In vivo, chondrocytes grown on both PGA and PLLA for 1-6 months maintained the three-dimensional (3-D) shapes of the original polymer scaffolds, appeared glistening white macroscopically, contained S-GAG and type II collagen, and closely resembled cartilage histologically. These studies demonstrate the feasibility of culturing isolated chondrocytes on biodegradable polymer scaffolds to regenerate 3-D neocartilage.