Release of TNF-α from lipopolysaccharide (LPS)-stimulated Kupffer cells in serum- and nutrient-free medium
Release of TNF-α from lipopolysaccharide (LPS)-stimulated Kupffer cells in serum- and nutrient-free medium
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DOI：
10.1023/a:1012856408531
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发表时间：
2001-10-01
期刊：
影响因子：
5.1
通讯作者：
Viebahn, R
中科院分区：
文献类型：
--
作者：
Zipfel, A;Schenk, M;Viebahn, R
在单核细胞/巨噬细胞中，LPS刺激通过LPS和血清组分LPS结合蛋白（LBP）与CD 14结合而发生。本研究旨在研究这种机制是否也发生在枯否细胞中。在RPMI培养基或无营养的Krebs-Henseleit（KH）缓冲液中，用LPS（0，100 ng/ml，10 μ g/ml）刺激大鼠枯否细胞长达8小时。一些孵育在没有血清的情况下进行，而在其他情况下提供血清。通过ELISA测量上清液的TNF-α浓度。Kupffer细胞的LPS刺激产生以下结果。在没有任何添加剂的KH中，释放了相当大量的TNF-α。在不含血清的RPMI中孵育导致释放的TNF-α是使用KH时的两倍。向RPMI中加入自体血清并不增加TNF-α反应。这些结果提供了证据表明，LPS刺激的库普弗细胞释放的TNF-α的相当大的一部分发生在血清-因此LBP-独立的方式。
In monocytes/macrophages LPS stimulation occurs by the binding of LPS and the serum component LPS-binding protein (LBP) to CD14. This study was conducted to investigate whether this mechanism also occurs in Kupffer cells. Rat Kupffer cells were stimulated for up to 8 h by LPS (0, 100 ng/ml, 10 mug/ml) in RPMI medium or in nutrient-free Krebs-Henseleit (KH) buffer. Some incubations were performed without serum, while in others serum was provided. TNF-alpha concentrations of the supernatants were measured by ELISA. LPS stimulation of Kupffer cells yielded the following results. In KH without any additives a considerable amount of TNF-alpha was released. Incubation in RPMI without serum caused twice as much TNF-alpha to be released as when KH was used. The addition of autologous serum to RPMI did not increase TNF-alpha response. These results provide evidence that a substantial part of TNF-alpha release by LPS-stimulated Kupffer cells occurs in a serum- and thus LBP-independent way.