EXPERIMENTAL MODIFICATION OF POSTNATAL CEREBELLAR GRANULE CELL-MIGRATION INVITRO
EXPERIMENTAL MODIFICATION OF POSTNATAL CEREBELLAR GRANULE CELL-MIGRATION INVITRO
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DOI：
10.1016/0006-8993(86)90872-3
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发表时间：
1986-07-09
期刊：
影响因子：
2.9
通讯作者：
SCHACHNER, M
中科院分区：
文献类型：
--
作者：
LINDNER, J;ZINSER, G;SCHACHNER, M
在细胞粘附分子L1和N-CAM的一价抗体片段存在下，培养[3H]胸苷脉冲标记颗粒细胞神经元在小脑叶外植体中的组织型迁移，抑制细胞迁移33.3 .+-。4.4%和13.9% +-。分别观察到2.1%。在L1抗原和N-CAM的单价抗体片段的等摩尔混合物存在下，没有观察到抑制细胞迁移的加性效应。L1、N-CAM和其他细胞表面糖蛋白共同的L2碳水化合物表位抗体对细胞迁移的影响与N-CAM抗体相似。针对细胞表面抗原M2的单克隆抗体和针对小鼠肝膜的多克隆抗体与所有小脑细胞类型的表面反应均未改变颗粒细胞的迁移行为。在神经氨酸酶、神经节苷脂结合毒素以及糖胺聚糖和糖胺聚糖降解酶的存在下培养外植体，也没有改变在所使用的培养条件下细胞迁移的程度。
Histotypic migration of [3H]thymidine pulse-labeled granule cell neurons in cerebellar folium explants were cultured in the presence of monovalent antibody fragments to cell adhesion molecules L1 and N-CAM, an inhibition of cell migration of 33.3 .+-. 4.4% and 13.9 .+-. 2.1%, respectively, was observed. In the presence of an equimolar mixture of monovalent antibody fragments to L1 antigen and N-CAM no additive effects in inhibition of cell migration were seen. Antibodies to the L2 carbohydrate epitope which is common to L1, N-CAM and other cell surface glycoproteins showed a similarly small effect on cell migration as antibodies to N-CAM. Monoclonal antibodies to cell surface antigen M2 and polyclonal antibodies to mouse liver membranes reacting with the surface of all cerebellar cell types did not alter the migratory behavior of granule cells. Cultivation of explants in the presence of neuraminidase, ganglioside binding toxins, as well as glycosaminoglycans and glycosaminoglycan degrading enzymes, also did not modify the extent of cell migration under the culture conditions used.