Assembly of the mitochondrial membrane system. Characterization of nuclear mutants of Saccharomyces cerevisiae with defects in mitochondrial ATPase and respiratory enzymes.
Assembly of the mitochondrial membrane system. Characterization of nuclear mutants of Saccharomyces cerevisiae with defects in mitochondrial ATPase and respiratory enzymes.
复制标题
线粒体膜系统的组装。
DOI：
10.1016/s0021-9258(19)40840-5
复制
发表时间：
1975
期刊：
影响因子：
--
通讯作者：
R. Needleman
中科院分区：
文献类型：
--
作者：
A. Tzagoloff;A. Akai;R. Needleman
描述了在细胞色素氧化酶、辅酶qh2 -细胞色素c还原酶和rutamycin敏感的atp酶中表现缺陷的酿酒酵母突变体。根据与细胞质微小测试菌株的互补和四分体的2:2分离，这些突变已被确定为核突变。遗传分析表明，辅酶qh2 -细胞色素c还原酶和细胞色素氧化酶突变体分别属于9个和10个不同的互补基团。根据线粒体细胞色素的吸收光谱，突变体也形成了不同的类别。其中两种atp酶突变体缺乏可检测到的F1 atp酶，而第三种可以合成F1，但不能将其整合到膜复合物中。后者突变体缺少一个线粒体合成的rutamycin敏感atp酶复合物亚基。
Mutants of Saccharomyces cereviaiae showing defects in cytochrome oxidase, coenzyme QH2-cytochrome c reductase, and rutamycin-sensitive ATPase are described. The mutations have been established to be nuclear, based on complementation with a cytoplasmic petite tester strain and 2:2 segregation of tetrads. Genetic analysis indicate the coenzyme QH2-cytochrome c reductase and cytochrome oxidase mutants fall into 9 and 10 different complementation groups, respectively. The mutants also form distinct classes based on absorption spectra of the mitochondrial cytochromes. Two of the ATPase mutants lack detectable F1 ATPase, while the third synthesizes F1 but does not integrate it into a membrane complex. The latter mutant is missing one of the mitochondrially synthesized subunits of the rutamycin-sensitive ATPase complex.