A highly sensitive monoclonal antibody based biosensor for quantifying 3-5 ring polycyclic aromatic hydrocarbons (PAHs) in aqueous environmental samples.
A highly sensitive monoclonal antibody based biosensor for quantifying 3-5 ring polycyclic aromatic hydrocarbons (PAHs) in aqueous environmental samples.
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DOI：
10.1016/j.sbsr.2016.02.003
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发表时间：
2016-03-01
影响因子：
5.3
通讯作者：
Unger, Michael A
中科院分区：
文献类型：
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作者：
Li, Xin;Kaattari, Stephen L;Unger, Michael A
基于单克隆抗体（mab）的免疫分析对多环芳烃（PAHs）的检测高度敏感，可以用于近实时的浓度测定。通过三步筛选程序开发了一种针对多环芳烃的敏感通用单抗，命名为2G8。它对3环至5环未取代多环芳烃及其常见的环境甲基化多环芳烃具有几乎一致的高灵敏度，IC50值在1.68 ~ 31马克杯/升（ppb）之间。2G8已成功应用于KinExA在线生物传感器系统，用于定量水环境样品中的3-5环多环芳烃。多环芳烃检测浓度低至0.2马克杯/升。此外，每个样品的分析只需要10分钟。为了评估基于2g8的生物传感器的准确性，比较了生物传感器和GC-MS分析的一系列环境样品中的总多环芳烃浓度。在大多数情况下，结果在方法之间产生了良好的相关性。这表明基于通用抗体2G8的生物传感器具有低成本、快速测定水样中多环芳烃的重要前景。
Immunoassays based on monoclonal antibodies (mAbs) are highly sensitive for the detection of polycyclic aromatic hydrocarbons (PAHs) and can be employed to determine concentrations in near real-time. A sensitive generic mAb against PAHs, named as 2G8, was developed by a three-step screening procedure. It exhibited nearly uniformly high sensitivity against 3-ring to 5-ring unsubstituted PAHs and their common environmental methylated PAHs, with IC50 values between 1.68-31 mug/L (ppb). 2G8 has been successfully applied on the KinExA Inline Biosensor system for quantifying 3-5 ring PAHs in aqueous environmental samples. PAHs were detected at a concentration as low as 0.2 mug/L. Furthermore, the analyses only required 10 min for each sample. To evaluate the accuracy of the 2G8-based biosensor, the total PAH concentrations in a series of environmental samples analyzed by biosensor and GC-MS were compared. In most cases, the results yielded a good correlation between methods. This indicates that generic antibody 2G8 based biosensor possesses significant promise for a low cost, rapid method for PAH determination in aqueous samples.