PCR amplification is more sensitive than tissue culture methods for Epstein-Barr virus detection in clinical material
PCR amplification is more sensitive than tissue culture methods for Epstein-Barr virus detection in clinical material
复制标题
DOI：
10.1099/0022-1317-78-12-3357
复制
发表时间：
1997-12-01
影响因子：
3.8
通讯作者：
Crawford, DH
中科院分区：
文献类型：
--
作者：
Haque, T;Crawford, DH
在这项研究中，我们比较了使用 PCR 和传统组织培养方法检测外周血单核细胞和咽喉洗液样本中的 Epstein-Barr 病毒 (EBV)。研究人群包括 29 名健康成年人和 20 名免疫功能低下的 EBV 血清阳性供者。结果显示，在两个供体组的咽喉清洗样本中，FCR 的 EBV 检出率显着高于组织培养方法（健康供体中 P < 0.01，免疫功能低下供体中 P < 0.009）以及免疫功能低下但非健康供体外周血中的 EBV 检出率（P < 0.008）。此外，当比较咽喉冲洗细胞沉淀物和上清液中的EBV DNA检出率时，细胞沉淀物获得了更高的阳性结果，在免疫功能低下组中达到了统计学显着性（P < 0.02）。同一捐献者的咽喉冲洗液阳性率与外周血阳性率之间没有发现相关性。
In this study we have compared the use of PCR and conventional tissue culture methods to detect Epstein-Barr virus (EBV) in peripheral blood mononuclear cells and throat wash samples. The study population included 29 healthy adult and 20 immunocompromised EBV-seropositive donors. The results show significantly higher EBV detection rates by FCR than the tissue culture methods in throat wash samples from both donor groups (P < 0.01 in healthy donors and P < 0.009 in the immunocompromised donors) and in peripheral blood from the immunocompromised but not from the healthy donors (P < 0.008). Furthermore, when EBV DNA detection rates in throat wash cell pellet and supernatant fluid were compared, a higher positive result was obtained with the cell pellets which reached statistical significance in the immunocompromised group (P < 0.02). No correlation was found between positivity in throat wash and peripheral blood from the same donors.