Circadian changes of D-alanine and related compounds in rats and the effect of restricted feeding on their amounts
Circadian changes of D-alanine and related compounds in rats and the effect of restricted feeding on their amounts
复制标题
DOI：
10.1016/j.jchromb.2008.04.004
复制
发表时间：
2008-11-01
影响因子：
3
通讯作者：
Zaitsu, Kiyoshi
中科院分区：
文献类型：
--
作者：
Morikawa, Akiko;Hamase, Kenji;Zaitsu, Kiyoshi
本文研究了哺乳动物固有的D-丙氨酸（D-Ala）的昼夜节律变化，并与L-Ala、其它D-氨基酸和几种激素的昼夜节律变化进行了比较。本文报道了用4-氟-7-硝基-2，1，3-苯并恶二唑（NBD-F）荧光衍生化后，用微型ODS柱和对映选择性柱相结合的二维高效液相色谱法测定大鼠血浆、胰腺和垂体前叶中D-丙氨酸的含量。昼夜两种生活习惯大鼠的D-Ala含量在睡眠期高，活动期低，首次表明D-Ala与动物的活动节律密切相关。相比之下，L-Ala和其他D-氨基酸没有显示出任何明显的昼夜节律变化。昼夜活动习惯大鼠血浆胰岛素水平与D-丙氨酸的昼夜变化呈负相关。结合我们以前的研究结果，即D-Ala定位于大鼠胰腺中的胰岛素分泌β细胞，这强烈地表明D-Ala与哺乳动物中的胰岛素具有某些功能关系。(c)2008 Elsevier B. V.保留所有权利。
The circadian changes of D-alanine (D-Ala), an intrinsic D-amino acid found in mammals,were investigated in rats with diurnal and nocturnal habits, and the profiles were compared to those of L-Ala, other D-amino acids and several hormones. Determination Of D-Ala in the rat plasma, pancreas and anterior pituitary gland was carried out using a sensitive and selective two-dimensional HPLC system combining a micro-ODS column and an enantioselective column after fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). The amount of D-Ala was high during the sleeping period and low during the active period in rats with both diurnal and nocturnal habits, indicating for the first time that the D-Ala is closely related to the activity rhythm of animals. In contrast, L-Ala and other D-amino acids did not show any clear circadian changes. The circadian change of D-Ala inversely correlated with that of the plasma insulin level in rats with both diurnal and nocturnal habits. Considered together with our previous findings that D-Ala is localized in the insulin secreting beta-cells in the rat pancreas, it is strongly suggested that D-Ala has some functional relationships to insulin in mammals. (c) 2008 Elsevier B.V. All rights reserved.