Rat estrogen receptor-α and -β, and progesterone receptor mRNA expression in various prostatic lobes and microdissected normal and dysplastic epithelial tissues of the Noble rats
Rat estrogen receptor-α and -β, and progesterone receptor mRNA expression in various prostatic lobes and microdissected normal and dysplastic epithelial tissues of the Noble rats
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DOI：
10.1210/en.139.1.424
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发表时间：
1998-01-01
期刊：
影响因子：
4.8
通讯作者：
Ho, SM
中科院分区：
文献类型：
--
作者：
Lau, KM;Leav, I;Ho, SM
采用半定量RT-PCR技术检测两种雌激素受体（ER）亚型，ER α和ER β，以及孕激素受体（PR）的转录本在诺布尔（NBL）大鼠前列腺各正常叶中是否存在差异表达和/或调控。我们发现，ER β mRNA在所有三个主要前列腺叶中均以相当高的水平存在：前列腺背侧（DP）、侧侧（LP）和腹侧（VP）。ER α mRNA在各叶中的表达水平较低，表达量由高到低依次为：LP>DP>VP。PR转录物的低表达与ER α mRNA的表达模式一致。用睾酮(T)加雌二醇-17 β (E-2) （T+E-2）或单独用T治疗的大鼠，VP、DP和LP中ER α、ER β和PR mRNA水平没有明显的变化，而用E-2治疗的大鼠，这三种转录物的表达普遍下降。然后，我们研究了这三种受体在T+E-2处理的大鼠前列腺背外侧正常上皮和发育不良上皮中的表达。在微解剖的发育不良上皮和正常上皮中发现了相当水平的ER β mRNA。相比之下，在发育不良的腺泡上皮样本中，PR mRNA的表达水平显著升高。在任何微解剖上皮样本中均未检测到ER α mRNA。本研究结果表明，PR mRNA表达上调可能通过ER β作用介导，参与了该动物模型中T+ e -2诱导的发育不良的发生。
Semiquantitative RT-PCR was used to determine if transcripts of the two estrogen receptor (ER) subtypes, ER alpha and ER beta, and the progesterone receptor (PR) are differentially expressed and/or regulated in the various normal lobes of the Noble (NBL) rat prostate. We found that ER beta mRNA was present at comparable, high levels in all three major prostatic lobes: dorsal (DP), lateral (LP) and ventral (VP) prostate. ER alpha mRNA was, however, expressed at low levels among the various lobes in the following descending order of abundance: LP>DP>VP. Expression of PR transcript was low and paralleled the expression pattern of ER alpha mRNA. Treatments of rats with testosterone (T) plus estradiol-17 beta (E-2) (T+E-2) or T alone induced no discernible alterations in ER alpha, ER beta, and PR mRNA levels in the VP, DP and LP, while those with E-2 caused a general decline in the expression of all three transcripts. We then studied the expression of the three receptors in the normal and dysplastic epithelium of the dorsolateral prostates (DLPs) of rats treated with T+E-2. Comparable levels of ER beta mRNA were found in microdissected dysplastic and normal epithelia. In contrast, significantly higher levels of PR mRNA were present in epithelial samples from dysplastic acini. ER alpha mRNA was not detected in any of the microdissected epithelial samples. Results from this study suggest that upregulation of PR mRNA expression, likely mediated via ER beta action, is involved in the genesis of T+E-2-induced dysplasia in this animal model.