Design and construction of a Bacillus amyloliquefaciens cell factory for hyaluronic acid synthesis from Jerusalem artichoke inulin.
Design and construction of a Bacillus amyloliquefaciens cell factory for hyaluronic acid synthesis from Jerusalem artichoke inulin.
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DOI：
10.1016/j.ijbiomac.2022.02.100
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发表时间：
2022-02
影响因子：
8.2
通讯作者：
Yanqin Ma;Yibin Qiu;Caiyuan Yu;Sha Li;Hong Xu
中科院分区：
文献类型：
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作者：
Yanqin Ma;Yibin Qiu;Caiyuan Yu;Sha Li;Hong Xu
Hyaluronic acid (HA), a high-value biomacromolecule, has wide applications in medical, cosmetic and food fields. Currently, employing the safe-grade microorganisms for de novo biosynthesis of HA from renewable substrates has become a promising alternative. In this study, we established aBacillus amyloliquefaciensstrain as platform for HA production from Jerusalem artichoke inulin. Firstly, the different HA and UDP-GlcUA synthase genes were introduced intoB. amyloliquefaciensto construct the HA synthesis pathway. Secondly, the byproduct polysaccharides were removed by knockingsacBandepsA-Ousing CRISPR/Cas9n system, resulting in a 13% increase in HA production. Finally, 2.89 g/L HA with a high molecular weight of 1.5 MDa was obtained after optimizing fermentation conditions and adding osmotic agents. This study demonstrates the engineeredB. amyloliquefacienscan effectively synthesize HA with Jerusalem artichoke inulin and provides a green route for HA production.
