Bacterial tactic response to silver nanoparticles.
Bacterial tactic response to silver nanoparticles.
复制标题
细菌对银纳米粒子的策略反应。
DOI：
10.1111/j.1758-2229.2011.00252.x
复制
发表时间：
2011
影响因子：
3.3
通讯作者：
Ortega-Calvo JJ
中科院分区：
文献类型：
--
作者：
Ortega-Calvo JJ
本研究以恶臭假单胞菌（PseudomonasputidaG 7）为代表，研究了其对银纳米粒子（AgNPs）的策略响应。该研究整合了AgNP的表面积和尺寸分布的表征，基于ATP产生的毒性测定，以及通过倒置毛细管测定（"池中化学"方法）评估驱避反应，以及通过计算机辅助运动分析确定的运动行为变化。我们的数据首次表明，纳米颗粒可以在低但与环境相关的亚致死浓度下引起细菌的负策略反应。通过化学池法获得的数据表明，细胞暴露于0.1 mg l− 1的两种不同粒径的AgNP制剂（最大直径分别≤ 100 nm和≤ 150 nm），在毛细管内产生的梯度中被排斥。 然而，细胞暴露于类似的低浓度的AgNO 3没有表现出任何可检测到的驱避反应，虽然它降低了20%的细胞活力，减少所造成的AgNPs。对暴露于AgNPs（0.2 mg l−1）的细胞的游泳行为进行计算机分析，发现与未暴露的对照组相比，转向事件显著增加，这是细菌排斥反应的特征。 更高的AgNPs浓度（高达100 mg l−1）也诱导了游泳行为的变化，尽管它们没有诱导任何可检测到的驱避反应，如通过池塘中的化学测定所确定的。 相比之下，AgNO 3在测试的广泛浓度范围内（0.001-100 mg l-1）未能诱导驱避游泳行为，并且在浓度高于0.1 mg l-1时引起细胞运动的显著抑制。 本文提供的证据表明，可能存在纳米级银诱导驱避反应的替代机制，这比大分子形式的银的毒性反应更直接，归因于离子形成和暴露。
In this study, we investigated the tactic response ofPseudomonas putidaG7, a representative soil bacterium, towards silver nanoparticles (AgNPs). The study integrated the characterization of surface area and size distribution of AgNPs, toxicity determinations, based on ATP production, and assessment of the repellent reaction by means of an inverted capillary assay (‘chemical‐in‐pond’ method), and changes in the motility behaviour determined by computer‐assisted motion analysis. Our data demonstrate, for the first time, that nanoparticles can elicit a negative tactic response in bacteria at low but environmentally relevant, sublethal concentrations. Data obtained by the chemical‐in‐pond method indicated that cells exposed to 0.1 mg l−1of two AgNPs preparations, differing in particle size (maximum diameter ≤ 100 nm and ≤ 150 nm respectively), were repelled in the gradients created inside the capillaries. However, cells exposed to similar low concentration of AgNO3did not demonstrate any detectable repellent response, although it reduced cell viability by 20%, a decrease comparable to that caused by AgNPs. Computer analysis of swimming behaviour of cells exposed to AgNPs (0.2 mg l−1) revealed a significant increase in turning events, as compared with unexposed controls, which is characteristic of bacterial repellent response. Greater AgNPs concentrations (up to 100 mg l−1) also induced changes in the swimming behaviour, although they did not induce any detectable repellent response as determined by the chemical‐in‐pond assays. In contrast, AgNO3failed to induce the repellent swimming behaviour within the wide range of concentrations tested (0.001–100 mg l−1), and caused a significant inhibition of cell motility at a concentration above 0.1 mg l−1. The evidence presented here suggests there are likely to be alternative mechanisms by which nano‐scale silver induces a repellent response, which is more direct than the toxic response of macro‐forms of silver, attributed to ion formation and exposure.
计算机辅助视频运动分析：研究运动性和趋化性的强大技术
DOI：
--
发表时间：
1995
期刊：
影响因子：
--
作者：
G. Lopez;R. K. Zimmer;C. R. Lovell
通讯作者：
C. R. Lovell
溶解电极表面金属离子的细菌化学引诱特性
DOI：
--
发表时间：
1996
期刊：
影响因子：
--
作者：
S. Sánchez;D. Schiffrin
通讯作者：
D. Schiffrin
影响因子：
2.9
作者：
Xu, XHN;Brownlow, WJ;Viola, JJ
通讯作者：
Viola, JJ