RNA tertiary interactions in the large ribosomal subunit: The A-minor motif
RNA tertiary interactions in the large ribosomal subunit: The A-minor motif
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DOI：
10.1073/pnas.081082398
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发表时间：
2001-04-24
影响因子：
11.1
通讯作者：
Steitz, TA
中科院分区：
文献类型：
--
作者：
Nissen, P;Ippolito, JA;Steitz, TA
分析的2.4埃分辨率的大核糖体亚基的晶体结构Haloarcula marismortui揭示了存在一个丰富的和普遍存在的结构基序，稳定RNA的三级和四级结构。这个基序被称为A-小基序，因为它涉及腺嘌呤的光滑小沟边缘插入相邻螺旋的小沟中，优选在C-G碱基对处，在那里它们与那些碱基对的2'OH中的一个或两个形成氢键。A-小模体稳定RNA螺旋之间的接触，环和螺旋之间的相互作用，以及连接和紧转弯的构象。结合在A位点或P位点的tRNA的3'末端腺嘌呤与23 S rRNA之间的相互作用是功能上重要的A-次要相互作用的例子。A-次要基序是迄今为止核糖体大亚基中最丰富的三级结构相互作用; 23 S和5S rRNA中的186个腺嘌呤参与，其中68个是保守的。它可能被证明是大RNA结构中普遍最重要的长程相互作用。
Analysis of the 2.4-Angstrom resolution crystal structure of the large ribosomal subunit from Haloarcula marismortui reveals the existence of an abundant and ubiquitous structural motif that stabilizes RNA tertiary and quaternary structures. This motif is termed the A-minor motif, because it involves the insertion of the smooth, minor groove edges of adenines into the minor groove of neighboring helices, preferentially at C-G base pairs, where they form hydrogen bonds with one or both of the 2' OHs of those pairs. A-minor motifs stabilize contacts between RNA helices, interactions between loops and helices, and the conformations of junctions and tight turns. The interactions between the 3' terminal adenine of tRNAs bound in either the A site or the P site with 23S rRNA are examples of functionally significant A-minor interactions. The A-minor motif is by far the most abundant tertiary structure interaction in the large ribosomal subunit; 186 adenines in 23S and 5S rRNA participate, 68 of which are conserved. It may prove to be the universally most important long-range interaction in large RNA structures.