Targeting and retention enhancement of quantum dots decorated with amino acids in an invertebrate model organism.
Targeting and retention enhancement of quantum dots decorated with amino acids in an invertebrate model organism.
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无脊椎动物模型生物中氨基酸修饰的量子点的靶向和保留增强
DOI：
10.1038/srep19802
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发表时间：
2016-01-25
影响因子：
4.6
通讯作者：
Xu SQ
中科院分区：
文献类型：
--
作者：
Xing R;Chen XD;Zhou YF;Zhang J;Su YY;Qiu JF;Sima YH;Zhang KQ;He Y;Xu SQ
量子点（QDs）在生物成像应用和靶向药物递送中的应用有望增加。然而，量子点在药物靶向方面的效率有待提高。在这里，我们发现与CdTe量子点相连的氨基酸显著提高了无脊椎动物家蚕的靶向转移效率和生物安全性。与裸QDs530相比，暴露24 h后，Ala-和gly -共轭QDs （QDs530-Ala和QDs530- gly）在循环系统中的传递效率分别提高了2.6±0.3和1.5±0.3倍，在靶组织丝腺中的传递效率分别提高了7.8±0.9和2.9±0.2倍。同时，偶联量子点在非靶组织脂肪体中的量分别减少（68.4±4.4）%和（46.7±9.1）%，进入非靶循环血细胞的速度显著降低。结果表明，与暴露于背血管后的QDs530相比，QDs530- ala在组织中的结构完整性更好，在血淋巴中的滞留时间更长。另一方面，QDs530-Ala显著降低了对血细胞、丝腺和脂肪体的毒性，降低了组织中活性氧（ROS）的数量。
The use of quantum dots (QDs) in biological imaging applications and targeted drug delivery is expected to increase. However, the efficiency of QDs in drug targeting needs to be improved. Here, we show that amino acids linked to CdTe QDs significantly increased the targeted transfer efficiency and biological safety in the invertebrate modelBombyx mori. Compared with bare QDs530, the transfer efficiency of Ala- and Gly-conjugated QDs (QDs530-Ala and QDs530-Gly) in circulatory system increased by 2.6 ± 0.3 and 1.5 ± 0.3 times and increased by 7.8 ± 0.9 and 2.9 ± 0.2 times in target tissue silk glands, respectively, after 24 h of QDs exposure. Meanwhile, the amount of conjugated QDs decreased by (68.4 ± 4.4)% and (46.7 ± 9.1)% in the non-target tissue fat body and the speed at which they entered non-target circulating blood cells significantly decreased. The resultant QDs530-Ala revealed a better structural integrity in tissues and a longer retention time in hemolymph than that of QDs530 after exposureviathe dorsal vessel. On the other hand, QDs530-Ala significantly reduced the toxicity to hemocytes, silk gland and fat body and reduced the amount of reactive oxygen species (ROS) in tissues.