Elderly women: Homocysteine reduction by short-term folic acid supplementation resulting in increased glucose concentrations and affecting lipid metabolism (C677T MTHFR polymorphism)
Elderly women: Homocysteine reduction by short-term folic acid supplementation resulting in increased glucose concentrations and affecting lipid metabolism (C677T MTHFR polymorphism)
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DOI：
10.1016/j.nut.2012.09.015
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发表时间：
2013-06-01
期刊：
影响因子：
4.4
通讯作者：
Gawecki, Jan
中科院分区：
文献类型：
--
作者：
Chmurzynska, Agata;Malinowska, Anna M.;Gawecki, Jan
目的：血清同型半胱氨酸水平显示个体间差异，并由营养因素，如B族维生素摄入量，以及年龄和遗传影响，如亚甲基四氢叶酸还原酶（MTHFR）基因的基因型决定。最近，一碳与脂质代谢的关系已被证明。因此，我们假设叶酸补充不仅会降低同型半胱氨酸浓度，而且会影响脂质代谢。本研究的目的是评估短期补充叶酸对同型半胱氨酸和脂质代谢的影响，在波兰妇女年龄超过60岁，不同的C677 T MTHFR genotype.Methods：122名志愿者补充叶酸400 μ g/d 8周。使用食物频率问卷评估叶酸摄入量。采用高效液相色谱法测定血清同型半胱氨酸水平。用Vitalab Flexor生化分析仪测量血清生物标志物。结果：研究组MTHFR基因型频率TT为0.14，CT为0.43，CC为0.43。基线时，平均叶酸和同型半胱氨酸浓度分别为12.16 +/- 0.23 ng/mL和7.94 +/- 0.3 μ mol/L。补充叶酸可降低血清同型半胱氨酸浓度。然而，膳食干预也导致葡萄糖浓度升高（P < 0.01）。T等位基因携带者的腰围和腰臀比均显著高于对照组（P <0.05）。结论：短期、低剂量叶酸补充可降低老年妇女血清同型半胱氨酸水平，但也可升高血糖水平。MTHFR基因C677 T多态性影响腰臀比和脂代谢。(c)2013 Elsevier Inc. All rights reserved.
Objective: Serum homocysteine levels show interindividual variation and are determined by nutritional factors, such as B-vitamin intake, and by age and genetic influences, such as the genotype of the methylenetetrahydrofolate reductase (MTHFR) gene. Recently, the relation between one-carbon and lipid metabolism has been shown. Therefore, we hypothesized that folic acid supplementation would not only decrease homocysteine concentrations but also affect lipid metabolism. The aim of the present study was to evaluate the impact of short-term folic acid supplementation on homocysteine and lipid metabolism in Polish women older than 60 y with different C677T MTHFR genotypes.Methods: One hundred twenty-two volunteers were supplemented with folic acid 400 mu g/d for 8 wk. Folate intake was assessed using a food-frequency questionnaire. The serum homocysteine level was analyzed using high-performance liquid chromatography. Serum biomarkers were measured with a Vitalab Flexor biochemical analyzer. MTHFR genotyping was performed using the polymerase chain reaction restriction fragment length polymorphism method.Results: In the studied group, the MTHFR genotype frequencies were 0.14 for TT, 0.43 for CT, 0.43 for CC carriers. At baseline, the average folic acid and homocysteine concentrations were 12.16 +/- 0.23 ng/mL and 7.94 +/- 0.3 mu mol/L, respectively. Folic acid supplementation lowered the serum homocysteine concentration. However, the dietary intervention also led to an increase in glucose concentrations (P < 0.01). The T-allele carriers had a larger waist circumference (P < 0.05) and a higher waist-to-hip ratio (P < 0.01).Conclusions: In elderly women, a short-term, low-dose folic acid supplementation lowered the serum homocysteine level but also increased glucose concentrations. The C677T MTHFR polymorphism affects the waist-to-hip ratio and lipid metabolism. (c) 2013 Elsevier Inc. All rights reserved.