Arthropod relationships revealed by phylogenomic analysis of nuclear protein-coding sequences
Arthropod relationships revealed by phylogenomic analysis of nuclear protein-coding sequences
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DOI：
10.1038/nature08742
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发表时间：
2010-02-25
期刊：
影响因子：
64.8
通讯作者：
Cunningham, Clifford W.
中科院分区：
文献类型：
--
作者：
Regier, Jerome C.;Shultz, Jeffrey W.;Cunningham, Clifford W.
节肢动物的显着古代，多样性和生态意义激发了许多试图解决其深层系统发育史的尝试，但混合了二十年的密集分子系统发育学的结果（1-7）。发现陆生昆虫（Hexapoda）与水生甲壳类似于与陆生cent虫和千足虫（2,8）（Myriapoda）（即使是特殊的成功）更紧密相关。更常见的是，基于有限的分类单元和基因样本的分析产生了不一致，弱支持且对分析条件高度敏感的结果（7,9,10）。在这里，我们提供了来自75种节肢动物物种的62个单拷贝核蛋白质编码基因的41千倍序列的可能性，贝叶斯和简约分析的可能性得到了强烈支持的结果。这些物种代表着每个主要的节肢动物谱系，再加上五种tardgrade和anychophorans作为外组。我们的结果强烈支持胰腺（Hexapoda Plus Crustacea），但也强烈支持传统的基于形态学的Mandibulata（11）（Myriapoda Plus Pancrustacea），而不是基于分子的Paradoxopoda（Myriapoda Plus plus chelicerata）（2,5,12）。除Hexapoda外，pancrustacea还包括“甲壳类动物”的三个主要谱系，每个谱系都涵盖了很大的形态差异。这些是寡菌（ostracods，mystacocarids，branchiurans和pentastomids），vericrustacea（malacostracans，thecostracans，thecostracans，copepods和branchiopods）和甲状腺质体（头孢菌素和消除剂）。最后，在pancrustacea中，我们将Xenocarida识别为六角洲长期以来的姊妹群体，结果证实了“甲壳类动物”不是单一的。这些结果为最大的动物门提供了一个统计上良好的系统发育框架，这代表了结束关于节肢动物关系的经常加热的，长达一个世纪的辩论的一步。
The remarkable antiquity, diversity and ecological significance of arthropods have inspired numerous attempts to resolve their deep phylogenetic history, but the results of two decades of intensive molecular phylogenetics have been mixed(1-7). The discovery that terrestrial insects (Hexapoda) are more closely related to aquatic Crustacea than to the terrestrial centipedes and millipedes(2,8) (Myriapoda) was an early, if exceptional, success. More typically, analyses based on limited samples of taxa and genes have generated results that are inconsistent, weakly supported and highly sensitive to analytical conditions(7,9,10). Here we present strongly supported results from likelihood, Bayesian and parsimony analyses of over 41 kilobases of aligned DNA sequence from 62 single-copy nuclear protein-coding genes from 75 arthropod species. These species represent every major arthropod lineage, plus five species of tardigrades and onychophorans as outgroups. Our results strongly support Pancrustacea (Hexapoda plus Crustacea) but also strongly favour the traditional morphology-based Mandibulata(11) (Myriapoda plus Pancrustacea) over the molecule-based Paradoxopoda (Myriapoda plus Chelicerata)(2,5,12). In addition to Hexapoda, Pancrustacea includes three major extant lineages of 'crustaceans', each spanning a significant range of morphological disparity. These are Oligostraca (ostracods, mystacocarids, branchiurans and pentastomids), Vericrustacea (malacostracans, thecostracans, copepods and branchiopods) and Xenocarida (cephalocarids and remipedes). Finally, within Pancrustacea we identify Xenocarida as the long-sought sister group to the Hexapoda, a result confirming that 'crustaceans' are not monophyletic. These results provide a statistically well-supported phylogenetic framework for the largest animal phylum and represent a step towards ending the often-heated, century-long debate on arthropod relationships.