Microbody proliferation and segregation cycle in the single-microbody alga Cyanidioschyzon merolae
Microbody proliferation and segregation cycle in the single-microbody alga Cyanidioschyzon merolae
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DOI：
10.1007/s004250050566
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发表时间：
1999-05
期刊：
影响因子：
4.3
通讯作者：
S. Miyagishima;R. Itoh;K. Toda;H. Kuroiwa;M. Nishimura;T. Kuroiwa
中科院分区：
文献类型：
--
作者：
S. Miyagishima;R. Itoh;K. Toda;H. Kuroiwa;M. Nishimura;T. Kuroiwa
研究了原始红藻Cyanidioschyzonmerolae微体的增殖周期。细胞同步与暗/光周期，和微体的形态及其与其他细胞器的相互作用进行了观察三维荧光显微镜，透射电子显微镜，和计算机辅助三维重建的系列薄切片。间期细胞中的微体是一个直径为0.3 μm的无核球体。在M期，微体通过一系列不规则的形状，在顺序杆，蠕虫，分支，H形和哑铃，和对称分裂发生在胞质分裂之前。微观体在M相中复制其体积，三维定量分析显示其表面积先增加后体积增加。微体在其整个增殖周期中接触叶绿体和叶绿体，除了在间期细胞中短暂地接触外，微体在一个阶段缠绕在叶绿体的分裂平面上。过氧化氢酶作为微体基质蛋白的标记物的免疫细胞化学标记显示，过氧化氢酶的复制与体积增加串联发生。虽然在微体分区中没有确定特定的装置，但我们确定了微体和微粒子之间直径约30-50 nm的电子致密装置，其可能在分离子微体中起作用。这些结果是第一个表征，以显示一个微体的形态学变化，在一个微体微生物学中没有增殖诱导底物，这已被用于许多研究，并清楚地表明，两个子微体产生的二元裂变的预先存在的微体。
The proliferation cycle of the microbody was studied in the primitive red algaCyanidioschyzon merolae, which contains one microbody per cell. Cells were synchronized with a dark/light cycle, and the morphology of the microbody and its interaction with other organelles were observed three-dimensionally by fluorescence microscopy, transmission electron microscopy, and computer-assisted three-dimensional reconstruction of serial thin sections. The microbody in interphase cells is a sphere of 0.3 μm in diameter without a core. In M-phase, the microbody passes through a series of irregular shapes, in the order rod, worm, branched, H-shaped and dumbbell, and symmetric fission occurs just before cytokinesis. The microbody duplicates its volume in M-phase and three-dimensional quantitative analysis revealed that its surface area increases before its volume does. The microbody touches the mitochondrion and the chloroplast throughout its proliferation cycle, except briefly in interphase cells, winding around the divisional plane of the mitochondrion at one phase. Immunocytochemical labeling of catalase as a marker of matrix proteins of the microbody revealed that the duplication of catalase occurs in tandem with the volume increase. While no specific apparatus was identified in the microbody divisional areas, we identified an electron-dense apparatus about 30–50 nm in diameter between the microbody and the mitochondrion that may play a role in segregating the daughter microbodies. These results are the first characterization to show the morphological changes of one microbody in a one-microbody alga without proliferation-inducing substrates, which have been used in many studies, and clearly show that two daughter microbodies arise by binary fission of the pre-existing microbody.