Methods for repeated recording in visual cortex of anesthetized and awake behaving infant monkeys.
Methods for repeated recording in visual cortex of anesthetized and awake behaving infant monkeys.
复制标题
重复记录麻醉和清醒行为幼猴视觉皮层的方法。
DOI：
10.1016/0165-0270(91)90171-u
复制
发表时间：
1991
影响因子：
3
通讯作者：
Rodman,HR
中科院分区：
文献类型：
--
作者：
Rodman,HR
本报告描述了在一氧化二氮麻醉和警觉行为状态下，从婴儿猕猴（年龄范围5周至7个月）的视觉皮层进行重复记录的方法。这些方法允许成功地收集单个单元的数据，从下颞叶皮层和其他地区的纵向方式从个别婴儿。对于麻醉实验，我们对手术准备和维持动物处于麻醉、人工通气状态的标准技术进行了修改，允许重复记录，同时确保动物的持续健康和正常生长。植入眼线圈后，年仅5周的猴子可以接受训练，在警报记录过程中执行固定任务，这些任务同样可以重复进行，并保持健康和发育。此外，从麻醉和清醒的记录会话的结果的比较表明，警报记录是优选的收集某些类型的数据，从视觉皮层在婴儿猴。
This report describes methods for making repeated recordings from visual cortex in infant macaque monkeys (age range 5 weeks to 7 months) both under nitrous oxide anesthesia and in an alert behaving state. These methods permitted successful collection of single-unit data from inferior temporal cortex and other areas in a longitudinal fashion from individual infants. For the anesthetized experiments, modifications of our standard techniques for surgical preparation and maintenance of the animal in an anesthetized, artificially ventilated state permitted repeated recording sessions while assuring the animal's continued health and normal growth. Following implantation with an eye coil, monkeys as young as 5 weeks could be trained to perform a fixation task during alert recording sessions which were likewise performed on a repeated basis with maintained health and development. Further, comparison of results from anesthetized and awake recording sessions indicates that alert recording is preferable for collecting certain types of data from visual cortex in infant monkeys.