Characterization of Enzymatic Antioxidants in the Lichen Ramalina lacera and Their Response to Rehydration
Characterization of Enzymatic Antioxidants in the Lichen Ramalina lacera and Their Response to Rehydration
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DOI：
10.1128/aem.71.11.6508-6514.2005
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发表时间：
2005-11
影响因子：
4.4
通讯作者：
L. Weissman;J. Garty;A. Hochman
中科院分区：
文献类型：
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作者：
L. Weissman;J. Garty;A. Hochman
摘要地衣是真菌和绿色藻类或蓝藻的生长缓慢的组合。这种共生关系形成了一个共同的叶状体，没有根或蜡质角质层，主要依赖于大气中输入的矿物质营养。大多数地衣的生活方式是由交替的干燥期与低代谢活性和水合作用，诱导其代谢的增加。我们以前已经表明，自然干燥的地衣Ramalina lacera的再水化导致光合作用的快速增加，并伴随着细胞内产生的活性氧和一氧化氮的爆发，以及水溶性抗氧化能力的瞬时下降。我们在这里报告酶抗氧化剂的R。lacera及其对补液反应。天然凝胶电泳的粗提物的R。lacera的超氧化物歧化酶（SOD）活性染色结果表明，该菌合成了4种Fe-SOD和4种Mn-SOD电子型，该菌合成了1种Cu/Zn-SOD和1种Mn-SOD，该菌和该藻分别合成了2种过氧化氢酶。此外，我们还检测了红球藻粗提物中谷胱甘肽还原酶和葡萄糖-6-磷酸脱氢酶的活性。lacera。再水化的菌体导致所有形式的SOD活性的下降，和总过氧化氢酶活性的短暂下降，以及抗氧化辅助酶谷胱甘肽还原酶和葡萄糖-6-磷酸脱氢酶的减少。
ABSTRACT Lichens are slow-growing associations of fungi and green algae or cyanobacteria. This symbiotic association forms a common thallus that does not possess roots or a waxy cuticle and depends mainly on atmospheric input of mineral nutrients. The lifestyle of most lichens is composed of alternating periods of desiccation with low metabolic activity and hydration that induces increase in their metabolism. We have previously shown that rehydration of the naturally desiccated lichen Ramalina lacera resulted in a rapid increase in photosynthesis and was accompanied by a burst of intracellular production of reactive oxygen species and nitric oxide, as well as a transient decrease in water-soluble antioxidant capacity. We report here on enzymatic antioxidants of R. lacera and their response to rehydration. Native gel electrophoresis of crude extracts of R. lacera stained for superoxide dismutase (SOD) activity revealed four Fe-SOD and four Mn-SOD electromorphs that are synthesized by the alga, a Cu/Zn-SOD and a Mn-SOD that are the product of the fungus, and two catalases synthesized one by the fungus and the other by the algae. In addition, we detected glutathione reductase and glucose-6-phosphate dehydrogenase activities in crude extracts of R. lacera. Rehydration of the thalli resulted in a decrease in SOD activity of all forms, and a transient decrease in total catalase activity, as well as a decrease in the antioxidant auxiliary enzymes glutathione reductase and glucose-6-phosphate dehydrogenase.