Increased neuronal activity fragments the Golgi complex
Increased neuronal activity fragments the Golgi complex
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DOI：
10.1073/pnas.1220978110
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发表时间：
2013-01-22
影响因子：
11.1
通讯作者：
Jan, Lily Yeh
中科院分区：
文献类型：
--
作者：
Thayer, Desiree A.;Jan, Yuh Nung;Jan, Lily Yeh
高尔基复合体对于细胞功能的许多方面是必不可少的，包括膜和分泌蛋白的运输和分选以及通过糖基化的翻译后修饰。我们观察到可逆的碎片的高尔基复合体在培养的海马神经元培养在过度兴奋的条件下。此外，在由于GABA（A）介导的抑制或撤销NMDA受体拮抗作用而导致的过度活动的培养神经元中发现高尔基体断裂。因此，在这项研究中建立的神经元活动过度和高尔基体结构之间的相互作用揭示了神经元活动对细胞器结构的先前未表征的影响。这一发现可能在高尔基体的蛋白质加工和运输以及对神经元信号传导的影响中具有重要作用。
The Golgi complex is essential for many aspects of cellular function, including trafficking and sorting of membrane and secretory proteins and posttranslational modification by glycosylation. We observed reversible fragmentation of the Golgi complex in cultured hippocampal neurons cultured in hyperexcitable conditions. In addition, Golgi fragmentation was found in cultured neurons with hyperactivity due to prolonged blockade of GABA(A)-mediated inhibition or withdrawal of NMDA receptor antagonism. The interplay between neuronal hyperactivity and Golgi structure established in this study thus reveals a previously uncharacterized impact of neuronal activity on organelle structure. This finding may have important roles in protein processing and trafficking in the Golgi as well as effects on neuronal signaling.