TRANSCRIPTIONAL DIFFERENCES OF THE HUMAN PAPILLOMAVIRUS TYPE-16 GENOME BETWEEN PRECANCEROUS LESIONS AND INVASIVE CARCINOMAS
TRANSCRIPTIONAL DIFFERENCES OF THE HUMAN PAPILLOMAVIRUS TYPE-16 GENOME BETWEEN PRECANCEROUS LESIONS AND INVASIVE CARCINOMAS
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DOI：
10.1128/jvi.62.3.1022-1027.1988
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发表时间：
1988-03-01
影响因子：
5.4
通讯作者：
SIMIZU, B
中科院分区：
文献类型：
--
作者：
SHIRASAWA, H;TOMITA, Y;SIMIZU, B
从一个活检样本中同时提取DNA和RNA，分析活检宫颈肿瘤中人乳头瘤病毒16型（HPV16）基因组DNA及其转录物。Southern印迹分析显示，20例宫颈上皮内瘤变（CIN）中有5例含有主要以游离体形式存在的HPV 16 DNA，7例浸润性癌中有2例含有整合到宿主DNA中的HPV 16基因组序列。北方（RNA）印迹分析显示，HPV16基因组序列在5个CIN和2个浸润性癌中具有转录活性。CIN中HPV16特异性转录物的模式是均匀的，主要转录物的大小为4.2、2.2、1.6和1.4个酶。然而，HPV 16特异性转录物在浸润性癌中的模式是可变的，并且与CIN不同，这表明转录模式的改变可能在恶性肿瘤的发展中起关键作用。
Human papillomavirus type 16 (HPV16) genome DNA and its transcripts in biopsied cervical neoplasias were analyzed by simultaneous extraction of DNA and RNA from one biopsied sample. Southern blot analysis revealed that 5 of 20 cervical intraepithelial neoplasias (CINs) contained HPV16 DNAs existing primarily as episomes and two of seven invasive carcinomas harbored HPV16 genome sequences integrated into the host DNA. Northern (RNA) blot analysis showed that the HPV16 genome sequences were transcriptionally active in the five CINs, as well as in the two invasive carcinomas. The pattern of HPV16-specific transcripts in the CINs was uniform, and the major transcripts were 4.2, 2.2, 1.6, and 1.4 kilobases in size. However, the pattern of HPV16-specific transcripts in the invasive carcinomas was variable and different for that in CINs, suggesting that the alteration of transcriptional pattern might play a key role in the development of malignancy.