Rapid detection of anti-H5 avian influenza virus antibody by fluorescence polarization immunoassay using a portable fluorescence polarization analyzer
Rapid detection of anti-H5 avian influenza virus antibody by fluorescence polarization immunoassay using a portable fluorescence polarization analyzer
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DOI：
10.1016/j.snb.2020.128160
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发表时间：
2020-07-15
影响因子：
8.4
通讯作者：
Tokeshi, Manabu
中科院分区：
文献类型：
--
作者：
Nishiyama, Keine;Takeda, Yohei;Tokeshi, Manabu
建立了一种快速、简便、选择性检测血清中抗H5亚型禽流感病毒抗体的荧光偏振免疫分析方法。制备了重组H5亚型AIV血凝素片段，并将其用荧光素标记，作为荧光免疫分析的标记抗原。将该标记的抗原与抗AIV血清（H1-H16亚型）混合，并使用便携式FP分析仪在微型装置上测量混合物的FP。发现FP与抗H5 AIV抗体（血清）的浓度成比例地增加，并且显著高于用其它血清获得的FP。证实了抗H5亚型AIV抗体的选择性检测。所需原始样品体积为2 μ L，分析时间在20 min内。该检测系统实现了高效的AIV现场诊断和监测。
A rapid, facile and selective detection of anti-H5 subtype avian influenza virus (AIV) antibody in serum by fluorescence polarization immunoassay (FPIA) was achieved. A fragment of recombinant H5 subtype AIV hemagglutinin was produced and labeled with fluorescein to use it as a labeled antigen in FPIA. This labeled antigen was mixed with anti-AIV sera (H1-H16 subtypes) and FP of the mixture was measured using a portable FP analyzer on a microdevice. It was found that FP increased in proportion to the concentration of anti-H5 AIV antibody (serum) and was significantly higher than FP obtained with the other sera. The selective detection of anti-H5 subtype AIV antibody was confirmed. The required volume of original sample was 2 mu L and analysis time was within 20 min. This detection system realizes an efficient on-site diagnosis and surveillance of AIV.