Amplification of chloroplast DNA using the polymerase chain reaction (PCR): a practical activity for secondary school students
Amplification of chloroplast DNA using the polymerase chain reaction (PCR): a practical activity for secondary school students
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使用聚合酶链式反应 (PCR) 扩增叶绿体 DNA：中学生的实践活动
DOI：
10.1080/00219266.2006.9656040
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
M. Bownes
中科院分区：
文献类型：
--
作者：
K. Hamilton;J. Barfoot;Kathleen E. Crawford;C. Simpson;P. Beaumont;M. Bownes
我们描述了一个聚合酶链反应（PCR）方案适用于中学和大学。该PCR方法可用于植物间遗传变异的研究。该方案利用的引物与不同植物属高度保守的核苷酸序列互补。叶绿体DNA扩增的区域位于这些保守序列之间，是非编码区域。这些非编码区显示出高频率的突变，并显示出相对较高的进化变化率。因此，可以使用该协议来探索植物之间的进化关系。甘蓝的结果可用于突出属内和属间的遗传相似性和差异。该协议是稳健的，适用于使用热循环器或一系列的水浴，从而使其可用于大多数学校和大学。
We describe a polymerase chain reaction (PCR) protocol suitable for use in secondary schools and colleges. This PCR protocol can be used to investigate genetic variation between plants. The protocol makes use of primers which are complementary to sequences of nucleotides that are highly conserved across different plant genera. The regions of chloroplast DNA amplified lie between these conserved sequences and are non-coding. These non-coding regions display a high frequency of mutations and show relatively high rates of evolutionary change. Thus it is possible to use the protocol to explore evolutionary relationships between plants. Results from Brassica oleracea can be used to highlight genetic similarity and differences within and across genera. The protocol is robust and is suitable for use either with a thermocycler or a series of water-baths, thus making it accessible for use in most schools and colleges.