MOLECULAR CHARACTERIZATION OF ROTAVIRUSES WITH DISTINCT GROUP ANTIGENS
MOLECULAR CHARACTERIZATION OF ROTAVIRUSES WITH DISTINCT GROUP ANTIGENS
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DOI：
10.1099/0022-1317-64-10-2093
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发表时间：
1983-01-01
影响因子：
3.8
通讯作者：
MCCRAE, MA
中科院分区：
文献类型：
--
作者：
PEDLEY, S;BRIDGER, JC;MCCRAE, MA
比较血清学和核酸研究进行轮状病毒分离株，有和没有原来的组抗原。间接免疫荧光法可将非典型分离株分为2组，每组具有其自身的不同组抗原，因此总共至少有3个不同的轮状病毒组。非典型分离株的基因组图谱分析再次将其分为2组，这与免疫荧光法获得的结果一致，且其中基因组片段的总体模式相似。使用单维末端指纹分析对3个病毒组之间的差异程度进行了更严格的检查。这表明3组中的病毒彼此不携带任何共同的基因组片段。因此，已经建立了允许将轮状病毒分为3组的实验标准。对进一步的非典型分离株进行分析，将确定是否有理由将轮状病毒分为流感病毒中所见的类型的许多单独的组。
Comparative serological and nucleic acid studies were carried out on rotavirus isolates, with and without the original group antigen. Indirect immunofluorescence allowed the atypical isolates to be divided into 2 groups each with its own distinct group antigen hence giving a total of at least 3 distinct rotavirus groups. Genome profile analysis of the atypical isolates again divided them into 2 groups which corresponded with those obtained by immunofluorescence and within which the overall pattern of genome segments was similar. A more rigorous examination of the degree of difference between the 3 virus groups was carried out using single-dimension terminal fingerprint analysis. This indicated that the viruses in the 3 groups did not carry any genome segments in common with each other. Thus, experimental criteria have been established which allow the division of rotaviruses into 3 groups. Analysis of further atypical isolates, as they become available, will establish whether the division of rotaviruses into a number of separate groups, of the type seen in influenza virus, is justified.