Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution.
Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution.
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DOI：
10.1364/oe.18.011181
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发表时间：
2010-05-24
期刊：
影响因子：
3.8
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Bishara W;Su TW;Coskun AF;Ozcan A
我们在芯片上展示了无透镜全息显微术，以实现~0.6 µm的空间分辨率，对应于~0.5的数值孔径，在~24 mm 2的大视场内。通过使用大孔径（~50 µm）的部分相干照明，我们获得了具有单位条纹放大率的物体的较低分辨率无透镜同轴全息图。对于每个无透镜全息图，传感器芯片处的像素大小限制了重构图像的空间分辨率。为了规避这一限制，我们实现了一个基于亚像素移位的超分辨率算法，以有效地恢复更高分辨率的数字全息图的对象，允许亚微米的空间分辨率，以实现整个传感器芯片的有效面积，这也是相当于成像视场（24平方毫米）由于单位放大率。我们证明了这种像素超分辨率方法的成功，通过成像图案化的透明基板，血液涂片样本，以及Caenoharditis Elegans。
We demonstrate lensfree holographic microscopy on a chip to achieve ~0.6 µm spatial resolution corresponding to a numerical aperture of ~0.5 over a large field-of-view of ~24 mm2. By using partially coherent illumination from a large aperture (~50 µm), we acquire lower resolution lensfree in-line holograms of the objects with unit fringe magnification. For each lensfree hologram, the pixel size at the sensor chip limits the spatial resolution of the reconstructed image. To circumvent this limitation, we implement a sub-pixel shifting based super-resolution algorithm to effectively recover much higher resolution digital holograms of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area, which is also equivalent to the imaging field-of-view (24 mm2) due to unit magnification. We demonstrate the success of this pixel super-resolution approach by imaging patterned transparent substrates, blood smear samples, as well as Caenoharbditis Elegans.