Accumulation of plant small heat-stress proteins in storage organs
Accumulation of plant small heat-stress proteins in storage organs
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DOI：
10.1007/s00425-002-0745-1
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发表时间：
2002-04
期刊：
影响因子：
4.3
通讯作者：
O. Lubaretz;U. zur Nieden
中科院分区：
文献类型：
--
作者：
O. Lubaretz;U. zur Nieden
植物小分子热应激蛋白（small heat-stress protein，sHSPs）不仅在高温下表达，而且在胚胎发生、小孢子发生和果实成熟等发育阶段也表达。本文提出了新的数据sHSPs在营养组织中的发生，其组织特异性分布，和细胞定位。我们在1年生悬铃木嫩枝中发现了sHSPs。和接骨木Sambucus nigraL.在藤本植物大叶马兜铃Aristolochia macrophylla Lamk.只在冬季。马兜铃属植物卷须中sHSPs定位于维管形成层细胞中。发芽后，在春天，这些蛋白质不再存在。此外，sHSPs在洋葱的块茎和鳞茎中积累，朱顶红（朱顶红），白花番红花，风信子，水仙，郁金香Tulipa gesnerianaL.，和马铃薯Solanum tuberosum L.（马铃薯）。在马铃薯块茎和水仙鳞茎中，胁迫蛋白定位于贮藏薄壁细胞的中央液泡中。为了获得更多关于贮藏蛋白和sHSPs之间可能的功能相关性的信息，监测烟草种子成熟和萌发过程中两种类型的蛋白质在烟草种子中的积累。sHSPs和球蛋白在花后第17天左右同时开始表达。在种子萌发过程中的sHSPs消失的存储蛋白平行。此外，在不含任何蛋白体或贮藏蛋白的转基因烟草胚中，未发现sHSPs，因此，sHSPs在多年生植物贮藏器官中的存在可能与贮藏蛋白的存在有关。
Plant small heat-stress proteins (sHSPs) have been shown to be expressed not only after exposure to elevated temperatures, but also at particular developmental stages such as embryogenesis, microsporogenesis, and fruit maturation. This paper presents new data on the occurrence of sHSPs in vegetative tissues, their tissue-specific distribution, and cellular localization. We have found sHSPs in 1-year-old twigs ofAcer platanoidesL. andSambucus nigraL. and in the lianaAristolochia macrophyllaLamk. exclusively in the winter months. In tendrils ofAristolochia, sHSPs were localized in vascular cambium cells. After budding, in spring, these proteins were no longer present. Furthermore, accumulation of sHSPs was demonstrated in tubers and bulbs ofAllium cepaL., Amaryllis(Hippeastrum hybridum hort.),Crocus albiflorusL.,Hyacinthus orientalisL.,Narcissus pseudonarcissusL.,Tulipa gesnerianaL., andSolanum tuberosumL. (potato). In potato tubers and bulb scales ofNarcissusthe stress proteins were localized in the central vacuoles of storage parenchyma cells. In order to obtain more information on a possible functional correlation between storage proteins and sHSPs, the accumulation of both types of protein in tobacco seeds during seed ripening and germination was monitored. The expression of sHSPs and globulins started simultaneously at about the 17th day after anthesis. During seed germination the sHSPs disappeared in parallel with the storage proteins. Furthermore, in embryos of transgenic tobacco plants, which do not contain any protein bodies or storage proteins, no sHSPs were found. Thus, the occurrence of sHSPs in perennial plant storage organs seems to be associated with the presence of storage proteins.